Determination of residual parachloroaniline produced by endodontic treatment after the use of 5% sodium hypochlorite and 2% chlorhexidine combined: an ex-vivo study
ABSTRACT: Aim: The objective of this work was to determine the concentrations of irrigating solutions and the residual content of parachloroaniline (PCA) formed after endodontic irrigation, using 5% NaOCl, 0.9% NaCl, 10% EDTA and 2% CHX 2%. Methodology Twenty premolars were used and 13 samples were collected per tooth from each of the treatment phases. Samples of: NaOCl, EDTA, CHX and PCA were quantified by UV and visible spectrophotometry. Results: Sodium hypochlorite decreased its concentration from 3.8% to 3.4% in phases 1 to 4. In phases 5, 6 and 7, residual NaOCl was measured with concentrations of 0.007%, 0.003% and 0.001% %. The concentration of EDTA decreased to 8.85% in phase 8. In phases 9, 10 and 11, irrigated with serum, EDTA was quantified with concentrations of 0.013% to 0.002% and NaOCl values of 0.0011% to 0, 0006%. In phases 12 and 13, CHX concentrations were 1.850% and 1.812% and PCA values were 0.0005% and 0.0007%. PCA formation occurred in presence of 2% CHX and residual NaClO and was detected colorimetrically in phases 12 and 13. Conclusions. During endodontic irrigation the concentration of 5% NaOCl decreases significantly in the first four phases and the concentrations of EDTA and CHX also decrease. There is PCA training in the last stages of the procedure.