dc.creator | Cartier R,Luis | |
dc.creator | Ramírez V,Eugenio | |
dc.creator | Galeno A,Héctor | |
dc.date | 1999-08-01 | |
dc.date.accessioned | 2019-05-02T21:10:32Z | |
dc.date.available | 2019-05-02T21:10:32Z | |
dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0034-98871999000800008 | |
dc.identifier.uri | http://revistaschilenas.uchile.cl/handle/2250/80949 | |
dc.description | Background: Tropical spastic paraparesis (TSP) is an endemic disease in Chile. In most countries, only 50% of patients are seropositive to HTLV-I. However, new studies suggest that seronegative TSP is also associated with HTLV-I. Aim: To describe cLinical and virological features of seronegative patients with TSP. Patients and methods: Seventy two Chilean patients with TSP, studied by cLinical, radiological and laboratory methods during 1998, are reported. The determination of antibodies to HTLV-I was accompLished by ELiSA, immunofluorescence and Western-blot analysis. Polymerase chain reaction for tax and 5'Ltr genes was made using primers SK 43-44, LTR1 and LTR6. Results: Thirty one patients were HTLV-I positive and 41 were negative. No cLinical, radiological or laboratory differences were observed between both groups. In seropositive patients, tax and 5'ltr viral gene sequences of the HTLV-I provirus were detected in DNA of peripheral blood mononuclear cells. In seronegative cases, sequences of tax gene were detected, exclusively, in 18 of 41 patients. Conclusions: These results confirm an association with HTLV-I infection in 43,9% of the TSP seronegative patients. These findings support the hypothesis that a defective provirus infects peripheral blood mononuclear cells in seronegative cases of TSP. The importance tax gene in the diagnosis of the TSP is also emphasized. | |
dc.format | text/html | |
dc.language | es | |
dc.publisher | Sociedad Médica de Santiago | |
dc.relation | 10.4067/S0034-98871999000800008 | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.source | Revista médica de Chile v.127 n.8 1999 | |
dc.subject | HTLV-I tax gene in TSP | |
dc.subject | HTLV-I antigens | |
dc.subject | Tropical spastic paraparesis | |
dc.title | El gen tax del virus linfotrópico humano tipo I en la identificación etiológica de la paraparesia espástica tropical: Estudio clínico, serológico y de polimerasa en cadena en 72 pacientes | |