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dc.creatorGonzález R,Margarita
dc.creatorSanz C,Ivette
dc.creatorRojas S,Nina
dc.creatorSilva F,Víctor
dc.creatorKirsten L,Lilian
dc.creatorBustamante C,Marcelo
dc.date1999-11-01
dc.date.accessioned2019-05-02T21:10:38Z
dc.date.available2019-05-02T21:10:38Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0034-98871999001100003
dc.identifier.urihttp://revistaschilenas.uchile.cl/handle/2250/81005
dc.descriptionBackground: Immune cells participate in the formation of atheromatous plate, however little is known about the effects of native or oxidatively modified lipoproteins on these cells. Aim: To study the effects of lipoproteins on in vitro mononuclear cell proliferation. Material and methods: Peripheral blood mononuclear cells were obtained from 10 patients with type 2 diabetes mellitus (aged 52 ± 9 years old with a disease duration of 8.2 ± 5.7 years and a mean glycosilated hemoglobin of 9.3 ± 2.2%) and 10 non diabetic healthy controls (aged 50.3 ± 7.1 years old). These were stimulated with phytohemagglutinin (PHA) alone or in the presence of native LDLS, malondialdehyde modified LDLs or glycated LDLs. Proliferation was measured as 3H-thymidine incorporation and expressed as Stimulation Index (SI). Results: SI of patients and healthy subjects, after PHA stimulation were similar: (57.5 ± 29.8 and 61.1 ± 23.5) respectively LDLs did not induce proliferation in neither group. Native LDLs produced a 98% inhibition of PHA induced proliferation. Malondialdehyde modified and glycated LDLs caused a 50% inhibition. The suppressive effect was maintained when lipoproteins were incorporated to culture media 60 min prior or after PHA stimulation. Conclusions: Lipoproteins inhibit in vitro PHA induced peripheral blood mononuclear cell proliferation both in diabetic and in non diabetic subjects.
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dc.languagees
dc.publisherSociedad Médica de Santiago
dc.relation10.4067/S0034-98871999001100003
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceRevista médica de Chile v.127 n.11 1999
dc.subjectAtherosclerosis
dc.subjectLipoproteins
dc.subjectLipoproteins, LDL
dc.subjectLymphocyte transformation
dc.titleEfecto inmunosupresor in vitro de las lipoproteínas de baja densidad


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