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dc.creatorMORENO,ISABEL M
dc.creatorPEREIRA,PAULO
dc.creatorFRANCA,SUSANA
dc.creatorCAMEÁN,ANA
dc.date2004-01-01
dc.date.accessioned2019-05-02T21:21:17Z
dc.date.available2019-05-02T21:21:17Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000300006
dc.identifier.urihttp://revistaschilenas.uchile.cl/handle/2250/81526
dc.descriptionThis paper describes the occurrence of toxic cyanobacteria along the Guadiana River over its course between Mérida and Badajoz (Extremadura, Spain). Water sampling for phytoplankton quantification and toxin analysis was carried out regularly between 1999 and 2001 in six different locations, including two shallow, slow-flowing river sites, two streamed river sites and two drinking water reservoirs. The cyanobacterial community differed significantly between these locations, especially during the summer. The predominant genera were Microcystis, Oscillatoria, Aphanizomenon and Anabaena. Using an ELISA assay the total microcystin contents of natural water samples from the most eutrophic locations ranged from 0.10 _ 21.86 mg mcyst-LR equivalent·L-1 in Valdelacalzada and 0.10-11.3 µg mcyst-LR equivalent·L-1 in Vitonogales, and a seasonal variation of toxin content was observed. The amount of microcystins produced by each strain was determined by ELISA assay and the detection and identification of microcystin variants of three toxic strains of Microcystis aeruginosa was performed by high performance liquid chromatography (HPLC). The analysis of microcystins of the cultured strains revealed that toxin production was variable among different strains of M. aeruginosa isolated either from different blooms or from the same bloom.
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dc.languageen
dc.publisherSociedad de Biología de Chile
dc.relation10.4067/S0716-97602004000300006
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceBiological Research v.37 n.3 2004
dc.subjectCyanobacteria
dc.subjectmicrocystins
dc.subjectGuadiana
dc.subjectSpain
dc.titleToxic cyanobacteria strains isolated from blooms in the Guadiana River (southwestern Spain)


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