Imaging single-channel calcium microdomains by total internal reflection microscopy

dc.creator	DEMURO,ANGELO
dc.creator	PARKER,IAN
dc.date	2004-01-01
dc.date.accessioned	2019-05-02T21:21:21Z
dc.date.available	2019-05-02T21:21:21Z
dc.identifier	https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400025
dc.identifier.uri	http://revistaschilenas.uchile.cl/handle/2250/81557
dc.description	The microdomains of Ca2+ in the cytosol around the mouth of open Ca2+ channels are the basic `building blocks' from which cellular Ca2+ signals are constructed. Moreover, the kinetics of local [Ca2+] closely reflect channel gating, so their measurement holds promise as an alternative to electrophysiological patch-clamp recording as a means to study single channel behavior. We have thus explored the development of optical techniques capable of imaging single-channel Ca2+ signals with good spatial and temporal resolution, and describe results obtained using total internal reflection fluorescence microscopy to monitor Ca2+ influx through single N-type channels expressed in Xenopus oocytes
dc.format	text/html
dc.language	en
dc.publisher	Sociedad de Biología de Chile
dc.relation	10.4067/S0716-97602004000400025
dc.rights	info:eu-repo/semantics/openAccess
dc.source	Biological Research v.37 n.4 2004
dc.subject	single-channel recording
dc.subject	calcium imaging
dc.subject	TIRFM
dc.subject	N-type Ca2+ channels
dc.title	Imaging single-channel calcium microdomains by total internal reflection microscopy