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A Rapid and Efficient Method for Purifying High Quality Total RNA from Peaches (Prunus persica) for Functional Genomics Analyses

dc.creatorMEISEL,LEE
dc.creatorFONSECA,BEATRIZ
dc.creatorGONZÁLEZ,SUSANA
dc.creatorBAEZA-YATES,RICARDO
dc.creatorCAMBIAZO,VERONICA
dc.creatorCAMPOS,REINALDO
dc.creatorGONZALEZ,MAURICIO
dc.creatorORELLANA,ARIEL
dc.creatorRETAMALES,JULIO
dc.creatorSILVA,HERMAN
dc.date2005-01-01
dc.date.accessioned2019-05-02T21:21:24Z
dc.date.available2019-05-02T21:21:24Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000100010
dc.identifier.urihttp://revistaschilenas.uchile.cl/handle/2250/81579
dc.descriptionPrunus persica has been proposed as a genomic model for deciduous trees and the Rosaceae family. Optimized protocols for RNA isolation are necessary to further advance studies in this model species such that functional genomics analyses may be performed. Here we present an optimized protocol to rapidly and efficiently purify high quality total RNA from peach fruits (Prunus persica). Isolating high-quality RNA from fruit tissue is often difficult due to large quantities of polysaccharides and polyphenolic compounds that accumulate in this tissue and co-purify with the RNA. Here we demonstrate that a modified version of the method used to isolate RNA from pine trees and the woody plant Cinnamomun tenuipilum is ideal for isolating high quality RNA from the fruits of Prunus persica. This RNA may be used for many functional genomic based experiments such as RT-PCR and the construction of large-insert cDNA libraries.
dc.formattext/html
dc.languageen
dc.publisherSociedad de Biología de Chile
dc.relation10.4067/S0716-97602005000100010
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceBiological Research v.38 n.1 2005
dc.subjectcDNA library
dc.subjectfruit
dc.subjectfruit trees
dc.subjectfunctional genomics
dc.subjectpeach
dc.subjectpolysaccharides
dc.subjectPrunus persica
dc.subjectRNA isolation
dc.subjectRosaceae
dc.subjectRT-PCR
dc.titleA Rapid and Efficient Method for Purifying High Quality Total RNA from Peaches (Prunus persica) for Functional Genomics Analyses


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