| dc.creator | BALDEIRAS,INÊS E | |
| dc.creator | SANTOS,ROSA M | |
| dc.creator | ROSÁRIO,LUÍS M | |
| dc.date | 2006-01-01 | |
| dc.date.accessioned | 2019-05-02T21:21:33Z | |
| dc.date.available | 2019-05-02T21:21:33Z | |
| dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000300014 | |
| dc.identifier.uri | http://revistaschilenas.uchile.cl/handle/2250/81681 | |
| dc.description | Thymeleatoxin (TMX), an activator of Ca2+-sensitive protein kinase C (cPKC) isoforms, was used to assess the PKC isoform specificity of cholinergic potentiation of glucose (11 mM)-induced pulsatile 5-HT/insulin release (PIR) from single mouse pancreatic islets. TMX (100 nM) and carbachol (Cch, 50 mM) enhanced PIR ~ 3-fold while reducing the underlying [Ca2+]i oscillations (duration and amplitude) by ~ 40-50%. Both effects were ablated by the specific PKC inhibitor bisindolylmaleimide and chronic TMX pretreatment. Cch also evoked an initial transient [Ca2+]i rise and surge of 5-HT release, which remained unaffected by chronic TMX pretreatment. It is concluded that the immediate cholinergic responses are insensitive to cPKC. In contrast, specific activation of a cPKC isoform mediates sustained cholinergic potentiation of glucose-induced insulin secretion. | |
| dc.format | text/html | |
| dc.language | en | |
| dc.publisher | Sociedad de Biología de Chile | |
| dc.relation | 10.4067/S0716-97602006000300014 | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Biological Research v.39 n.3 2006 | |
| dc.subject | Cytosolic free Ca2+ concentration | |
| dc.subject | 5-HT amperometry | |
| dc.subject | islet of Langerhans | |
| dc.subject | protein kinase C | |
| dc.subject | pulsatile insulin release | |
| dc.subject | thymeleatoxin | |
| dc.title | Protein kinase C isoform specificity of cholinergic potentiation of glucose-induced pulsatile 5-HT/ insulin release from mouse pancreatic islets | |
