| dc.creator | VERGARA,JULIO L | |
| dc.creator | DIFRANCO,MARINO | |
| dc.date | 2006-01-01 | |
| dc.date.accessioned | 2019-05-02T21:21:34Z | |
| dc.date.available | 2019-05-02T21:21:34Z | |
| dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000300017 | |
| dc.identifier.uri | http://revistaschilenas.uchile.cl/handle/2250/81687 | |
| dc.description | The effects of caffeine on the process of excitation-contraction coupling in amphibian skeletal muscle fibers were investigated using the confocal spot detection technique. This method permits to carefully discriminate between caffeine effects on the primary sources of Ca2+ release at the Z-lines where the triads are located and secondary actions on other potential Ca Release sources. Our results demonstrate that 0.5 mM caffeine potentiates and prolongs localized action-potential evoked Ca2+ transients recorded at the level of the Z-lines, but that 1mM only prolongs them. The effects at both doses are reversible. At the level of the M-line, localized Ca2+ transients displayed more variability in the presence of 1 mM caffeine than in control conditions. At this dose of caffeine, extra-junctional sources of Ca2+ release also were observed occasionally. | |
| dc.format | text/html | |
| dc.language | en | |
| dc.publisher | Sociedad de Biología de Chile | |
| dc.relation | 10.4067/S0716-97602006000300017 | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Biological Research v.39 n.3 2006 | |
| dc.subject | excitation-contraction coupling | |
| dc.subject | caffeine | |
| dc.subject | confocal spot detection | |
| dc.subject | calcium transients | |
| dc.subject | frog skeletal muscle | |
| dc.subject | calcium microdomains | |
| dc.title | Modulation by caffeine of calcium-release microdomains in frog skeletal muscle fibers | |
