| dc.creator | POLANCO,RUBÉN | |
| dc.creator | CANESSA,PAULO | |
| dc.creator | RIVAS,ALEXIS | |
| dc.creator | LARRONDO,LUIS F | |
| dc.creator | LOBOS,SERGIO | |
| dc.creator | VICUÑA,RAFAEL | |
| dc.date | 2006-01-01 | |
| dc.date.accessioned | 2019-05-02T21:21:34Z | |
| dc.date.available | 2019-05-02T21:21:34Z | |
| dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000500007 | |
| dc.identifier.uri | http://revistaschilenas.uchile.cl/handle/2250/81703 | |
| dc.description | In this report we describe the isolation and characterization of a gene encoding the transcription factor Acel (Activation protein of cup 1 Expression) in the white rot fungus Phanerochaete chrysosporium. Pc-acel encodes a predicted protein of 633 amino acids containing the copper-fist DNA binding domain typically found in fungal transcription factors such as Acel, Macl and Haal from Saccharomyces cerevisiae. The Pc-acel gene is localized in Scaffold 5, between coordinates 220841 and 222983. A S. cerevisiae acel null mutant strain unable to grow in high-copper medium was fully complemented by transformation with the cDNA of Pc-acel. Moreover, Northern blot hybridization studies indicated that Pc-acel cDNA restores copper inducibility of the yeast cup 1 gene, which encodes the metal-binding protein metallothionein implicated in copper resistance. To our knowledge, this is first report describing an Acel transcription factor in basidiomycetes | |
| dc.format | text/html | |
| dc.language | en | |
| dc.publisher | Sociedad de Biología de Chile | |
| dc.relation | 10.4067/S0716-97602006000500007 | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Biological Research v.39 n.4 2006 | |
| dc.subject | Acel | |
| dc.subject | basidiomycete | |
| dc.subject | copper | |
| dc.subject | Phanerochaete chrysosporium | |
| dc.title | Cloning and functional characterization of the gene encoding the transcription factor Acel in the basidiomycete Phanerochaete chrysosporium | |
