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dc.creatorZIMMERMANN,KATRIN
dc.creatorHOLTZ,MARLIS
dc.creatorPHI-VAN,LOC
dc.date2007-01-01
dc.date.accessioned2019-05-02T21:21:37Z
dc.date.available2019-05-02T21:21:37Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000200009
dc.identifier.urihttp://revistaschilenas.uchile.cl/handle/2250/81750
dc.descriptionWe used a rapid and simple protocol using lysolecithin for mapping HS sites in vivo. The protocol is based on partial digestion with DNase I of exponentially growing cells following permeabilization by short treatment with lysolecithin. Using this protocol, we analyzed the chromatin structure of the region surrounding two overlapping elements, an origin of bidirectional DNA replication and the GAS41 promoter, in chicken myelomonocytic HD11 cells arrested in G0, G0 and S phases as well as at the G0/S border. The results show that the chromatin of this region became more nuclease sensitive when cells were arrested in G0 phase and that this change in chromatin structure was reversible after the cells began to enter S phase
dc.formattext/html
dc.languageen
dc.publisherSociedad de Biología de Chile
dc.relation10.4067/S0716-97602007000200009
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceBiological Research v.40 n.2 2007
dc.subjectchromatin structure
dc.subjectorigin of bidirectional replication
dc.subjectLysozyme
dc.subjectGAS41
dc.subjectpromoter
dc.titleThe chromatin structure of the lysozyme GAS41 origin of DNA replication changes during the cell cycle


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