Show simple item record

dc.creatorCUDDON,PAUL
dc.creatorBOOTMAN,MARTIN D
dc.creatorRICHARDS,GILLIAN R
dc.creatorSMITH,ALISON J
dc.creatorSIMPSON,PETER B
dc.creatorRODERICK,H LLEWELYN
dc.date2008-01-01
dc.date.accessioned2019-05-02T21:21:44Z
dc.date.available2019-05-02T21:21:44Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000200008
dc.identifier.urihttp://revistaschilenas.uchile.cl/handle/2250/81864
dc.descriptionNeurons are a diverse cell type exhibiting hugely different morphologies and neurotransmitter specifications. Their distinctive phenotypes are established during differentiation from pluripotent precursor cells. The signalling pathways that specify the lineage down which neuronal precursor cells differentiate remain to be fully elucidated. Among the many signáis that impinge on the differentiation of neuronal cells, cytosolic calcium (Ca2+) has an important role. However, little is known about the nature of the Ca2+ signáis involved in fate choice in neuronal precursor cells, or their sources. In this study, we show that activation of either muscarinic or platelet-derived growth factor (PDGF) receptors induces a biphasic increase in cytosolic Ca2+ that consists of reléase from intracellular stores followed by sustained entry across the plasma membrane. For both agonists, the prolonged Ca2+ entry occurred via a store-operated pathway that was pharmacologically indistinguishable from Ca2+ entry initiated by thapsigargin. However, muscarinic receptor-activated Ca2+ entry was inhibited by siRNA-mediated knockdown of TRPC6, whereas Ca2+ entry evoked by PDGF was not. These data provide evidence for agonist-specific activation of molecularly distinct store-operated Ca2+ entry pathways, and raise the possibility of privileged communication between these Ca2+ entry pathways and downstream processes.
dc.formattext/html
dc.languageen
dc.publisherSociedad de Biología de Chile
dc.relation10.4067/S0716-97602008000200008
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceBiological Research v.41 n.2 2008
dc.subjectCa2+
dc.subjectPDGF
dc.subjectmethacholine
dc.subjectTRPC6
dc.subjectSOCE
dc.subjecthuman neurosphere-derived cells
dc.titleMethacholine and PDGF activate store-operated calcium entry in neuronal precursor cells via distinct calcium entry channels


This item appears in the following Collection(s)

Show simple item record