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dc.creatorALATZAS,ANASTASIOS
dc.creatorSREBREVA,LJUBA
dc.creatorFOUNDOULI,ATHINA
dc.date2008-01-01
dc.date.accessioned2019-05-02T21:21:44Z
dc.date.available2019-05-02T21:21:44Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000200010
dc.identifier.urihttp://revistaschilenas.uchile.cl/handle/2250/81866
dc.descriptionAlthough several linker histone variants have been studied in both animal and plant organisms, little is known about their distribution during processes that involve alterations in chromatin function, such as differentiation, dedifferentiation and hormone treatment. In this study, we identified linker histone variants by using specific anti-histone Hl antibodies. Each variant's ratio to total Hl in the three developmental zones of maize (Zea mays L.) root and in callus cultures derived from them was estimated in order to define possible alterations either during plant cell differentiation or during their dedifferentiation. We also evaluated linker histone variants' ratios in the developmental zones of maize roots treated with auxin in order to examine the effects of exogenous applied auxin to linker histone variant distribution. Finally, immunohistochemical detection was used to identify the root tissues containing each variant and correlate them with the physiological status of the plant cells. According to the results presented in this study, linker histone variants' ratios are altered in the developmental zones of maize root, while they are similar to the meristematic zone in samples from callus cultures and to the differentiation zone in samples from roots treated with auxin. We propose that the alterations in linker histone variants' ratios are correlated with plant cell differentiation and dedifferentiation.
dc.formattext/html
dc.languageen
dc.publisherSociedad de Biología de Chile
dc.relation10.4067/S0716-97602008000200010
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceBiological Research v.41 n.2 2008
dc.subjectcallus culture
dc.subjectlinker histone
dc.subjectplant cell differentiation
dc.subjectplant hormone
dc.subjectZea mays
dc.titleDistribution of linker histone variants during plant cell differentiation in the developmental zones of the maize root, dedifferentiation in callus culture after auxin treatment


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