| dc.creator | MAINARDES,RUBIANA M | |
| dc.creator | PALMIRA D. GREMIÃO,MARIA | |
| dc.date | 2009-01-01 | |
| dc.date.accessioned | 2019-05-02T21:21:50Z | |
| dc.date.available | 2019-05-02T21:21:50Z | |
| dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000300010 | |
| dc.identifier.uri | http://revistaschilenas.uchile.cl/handle/2250/81959 | |
| dc.description | The development and validation of a simple and accurate method based on HPLC with ultraviolet detection for the quantification of zidovudine in rat plasma and its application to a pharmacokinetic study following a single intranasal dose zidovudine is described. Zidovudine was extracted from the plasma using a single-step deproteinization. Chromatographic separation of zidovudine from interfering components was achieved with a C-18 reverse phase column, a mobile phase consisting of a mixture of sodium acetate buffer (55mM) with pH adjusted to 7.0 and acetonitrile (91:9 v/v) and UV detection set at 265 nm. The method was linear from 100 to 10000 ng.mL"¹ (r² > 0.9995), and zidovudine had a mean recovery from plasma of 92.8%. The coefficient of variation of inter-day and intra-day quality control samples was less than 15%. After a single intranasal dose of zidovudine administered to rats, pharmacokinetic parameters (AUC0 24, Cmax, t , t1/2) were determined. The proposed method was found to be simple, specific, accurate, and precise and could be applied to the quantitative analysis of clinical pharmacokinetic studies of zidovudine in rats. | |
| dc.format | text/html | |
| dc.language | en | |
| dc.publisher | Sociedad de Biología de Chile | |
| dc.relation | 10.4067/S0716-97602009000300010 | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Biological Research v.42 n.3 2009 | |
| dc.subject | RP-HPLC | |
| dc.subject | validation | |
| dc.subject | AZT | |
| dc.subject | pharmacokinetics | |
| dc.subject | intranasal delivery | |
| dc.title | Reversed phase HPLC determination of zidovudine in rat plasma and its pharmacokinetics after a single intranasal dose administration | |
