| dc.creator | ROJAS-DOTOR,SARA | |
| dc.creator | PÉREZ-RAMOS,JULIA | |
| dc.creator | GIMÉNEZ-SCHERER,JUAN ANTONIO | |
| dc.creator | BLANCO-FAVELA,FRANCISCO | |
| dc.creator | RICO-ROSILLO,GUADALUPE | |
| dc.date | 2009-01-01 | |
| dc.date.accessioned | 2019-05-02T21:21:51Z | |
| dc.date.available | 2019-05-02T21:21:51Z | |
| dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000400003 | |
| dc.identifier.uri | http://revistaschilenas.uchile.cl/handle/2250/81968 | |
| dc.description | Entamoeba histolytica produces Monocyte Locomotion Inhibitory Factor (MLIF), which may contribute to the delayed inflammation observed in amoebic hepatic abscesses. Leukocytes are affected through the modulation of cytokine expression and/or production. We evaluated the effects of MLIF on the activation and production of intracellular cytokines in human CD4+ T lymphocytes by flow cytometry. Cells were stimulated for 24 h with PMA, MLIF, or PMA+MLIF. Cellular activation was measured using anti-CD69. Th1/Th2 production was studied by the expression of intracellular cytokines and cytokine/chemokine receptors. MLIF increased CD69 and induced the over-expression of the IL-lß, IFN-γ, IL-2, IL-4, and IL-10 intracellular cytokines; PMA+MLIF inhibited Th1 cytokine (IFN-γ) and increased Th2 cytokines (IL-4 and IL-10). The co-expression of the cytokine and chemokine receptors IFN-γ/CCR5 and IL-1ß/CCR5 was inhibited by PMA+MLIF and Th2 co-expression was increased. MLIF effects varied depending on the conditions. MLIF alone activated the Th1 and Th2 cytokines and cytokine/receptor expression; however, PMA+MLIF increased the expression of Th2 but inhibited it in Th1. | |
| dc.format | text/html | |
| dc.language | en | |
| dc.publisher | Sociedad de Biología de Chile | |
| dc.relation | 10.4067/S0716-97602009000400003 | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Biological Research v.42 n.4 2009 | |
| dc.subject | Amebic hepatic abscess (AHA) | |
| dc.subject | CC chemokine receptors (CCR4, CCR5) | |
| dc.subject | monocyte locomotion inhibitory factor (MLIF) | |
| dc.subject | Entamoeba histolytica (E. histolytica) | |
| dc.subject | 1-phorbol-12 myristate-13 acetate (PMA) | |
| dc.subject | interleukin (IL) | |
| dc.subject | T helper 1 lymphocyte (Th1) | |
| dc.subject | T helper 2 lymphocyte Th2) | |
| dc.title | Effect of the monocyte locomotion inhibitory factor (MLIF) produced by E. histolityca on cytokines and chemokine receptors in T CD4+ lymphocytes | |
