Show simple item record

Ethyl acetate fraction from Cudrania tricuspidata inhibts IL-1β-induced rheumatoid synovial fibroblast proliferation and MMPs, COX-2 and PGE2 production

dc.creatorLee,Eun-Gyeong
dc.creatorLee,Sang-ll
dc.creatorChae,Han-Jung
dc.creatorPark,Seoung Ju
dc.creatorLee,Yong Chul
dc.creatorYoo,Wan-Hee
dc.date2010-01-01
dc.date.accessioned2019-05-02T21:21:55Z
dc.date.available2019-05-02T21:21:55Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000200010
dc.identifier.urihttp://revistaschilenas.uchile.cl/handle/2250/82049
dc.descriptionObjectives: The objective of this study is to determine the effects of Ethyl acetate fraction from Cudrania tricuspidata (EACT) on the interleukin-1b (IL-1b)-induced proliferation of rheumatoid synovial fbroblasts (RASFs) and production of matrix metalloproteinases (MMPs), cyclooxygenase (COX) and prostaglandin E2 (PGE2) by RASFs. Materials and Methods: The proliferation of RASFs was evaluated with CCK-8 reagent in the presence of IL-1b with/without EACT. The expression of MMPs, TIMP-1, COXs, PGE2 and intracellular MAPK signalings, including p-ERK, p-p38, p-JNK and NF-kB were examined by immunoblotting or semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) and ELISA in conditions as described above. Results: EACT inhibits IL-1β-induced proliferation of RASFs and MMP-1, 3, COX-2 mRNA and protein expression, PGE2 production induced with IL-1b. EACT also inhibits the phosphorylation of ERK-1/2, p38, JNK and activation of NF-kB by IL-1b. Conclusions: These results suggest that EACT might be involved in synovial fbroblast proliferation and MMPs, COX-2, and PGE2 production, which are involved in joint destruction in rheumatoid arthritis (RA), indicating that this might be a new therapeutic modality for management of rheumatoid arthritis.
dc.formattext/html
dc.languageen
dc.publisherSociedad de Biología de Chile
dc.relation10.4067/S0716-97602010000200010
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceBiological Research v.43 n.2 2010
dc.subjectCudrania tricuspidata
dc.subjectCOX
dc.subjectIL-1b
dc.subjectMMPs
dc.subjectPGE2
dc.subjectRheumatoid Arthritis (RA)
dc.titleEthyl acetate fraction from Cudrania tricuspidata inhibts IL-1β-induced rheumatoid synovial fibroblast proliferation and MMPs, COX-2 and PGE2 production


This item appears in the following Collection(s)

Show simple item record