| dc.creator | Graça-de Souza,Viviane K | |
| dc.creator | Monteiro-Góes,Viviane | |
| dc.creator | Manque,Patrício | |
| dc.creator | Souza,Tatiana A.C.B | |
| dc.creator | Corrêa,Paulo R.C. | |
| dc.creator | Buck,Gregory A | |
| dc.creator | Ávila,Andréa R | |
| dc.creator | Yamauchi,Lucy M | |
| dc.creator | Pinge-Filho,Phileno | |
| dc.creator | Goldenberg,Samuel | |
| dc.creator | Krieger,Marco A | |
| dc.creator | Yamada-Ogatta,Sueli F | |
| dc.date | 2010-01-01 | |
| dc.date.accessioned | 2019-05-02T21:21:55Z | |
| dc.date.available | 2019-05-02T21:21:55Z | |
| dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000200011 | |
| dc.identifier.uri | http://revistaschilenas.uchile.cl/handle/2250/82050 | |
| dc.description | The genus Phytomonas comprises trypanosomatids that can parasitize a broad range of plant species. These fagellates can cause diseases in some plant families with a wide geographic distribution, which can result in great economic losses. We have demonstrated previously that Phytomonas serpens 15T, a tomato trypanosomatid, shares antigens with Trypanosoma cruzi, the agent of human Chagas disease. Herein, two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS) were used to identify proteins of P. serpens 15T that are recognized by sera from patients with Chagas disease. After 2D-electrophoresis of whole-cell lysates, 31 peptides were selected and analyzed by tandem mass spectrometry. Twenty-eight polypeptides were identifed, resulting in 22 different putative proteins. The identifed proteins were classifed into 8 groups according to biological process, most of which were clustered into a cellular metabolic process category. These results generated a collection of proteins that can provide a starting point to obtain insights into antigenic cross reactivity among trypanosomatids and to explore P. serpens antigens as candidates for vaccine and immunologic diagnosis studies. | |
| dc.format | text/html | |
| dc.language | en | |
| dc.publisher | Sociedad de Biología de Chile | |
| dc.relation | 10.4067/S0716-97602010000200011 | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Biological Research v.43 n.2 2010 | |
| dc.subject | Phytomonas serpens | |
| dc.subject | Trypanosoma cruzi | |
| dc.subject | antigenic cross-reactivity | |
| dc.subject | trypanosomatids | |
| dc.title | Sera of Chagasic patients react with antigens from the tomato parasite Phytomonas serpens | |
