| dc.creator | Pino-Lagos,Karina | |
| dc.creator | Michea,Paula | |
| dc.creator | Sauma,Daniela | |
| dc.creator | Alba,Andrea | |
| dc.creator | Morales,Jorge | |
| dc.creator | Bono,María Rosa | |
| dc.creator | Fierro,Alberto | |
| dc.creator | Rosemblatt,Mario | |
| dc.date | 2010-01-01 | |
| dc.date.accessioned | 2019-05-02T21:21:56Z | |
| dc.date.available | 2019-05-02T21:21:56Z | |
| dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000300010 | |
| dc.identifier.uri | http://revistaschilenas.uchile.cl/handle/2250/82072 | |
| dc.description | One of the mechanisms for generation of tolerance involves immature dendritic cells (DCs) and a subpopulation of regulatory CD4+ CD25+ T lymphocytes (T REG). The purpose of this work was to analyze how Cyclosporine A (CsA), a widely used immunosuppressive drug, may affect T REG proliferation. Purified and activated murine DCs obtained from bone marrow precursors differentiated with rGMCSF were co-cultured with purified CFSE-labeled T REG from OTII mice, and their phenotype and proliferation analyzed by flow cytometry. Our data indicate that DCs differentiated in the presence of CsA show an altered phenotype, with a lower expression of MHC-II and a lower activating capacity. Additionally, these CsA-treated DCs show decreased production of IL-2 and IL-12 and increased IL-10 secretion when stimulated with LPS, indicating an effect on the polarization of the immune response. Interestingly, CsA-treated DCs show an anti-tolerogenic effect since they reduce the proliferation of T REG cells from 72 to 47%. Further inhibition to a 24% of T REG proliferation was obtained as a direct effect of CsA on T REG. In conclusion, the anti-tolerogenic effect of CsA should be considered in the planning of immunosuppression in the context of clinical transplantation. | |
| dc.format | text/html | |
| dc.language | en | |
| dc.publisher | Sociedad de Biología de Chile | |
| dc.relation | 10.4067/S0716-97602010000300010 | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Biological Research v.43 n.3 2010 | |
| dc.subject | CD4+CD25+ regulatory T cell | |
| dc.subject | Cyclosporine A | |
| dc.subject | dendritic cells | |
| dc.subject | transplant tolerance | |
| dc.title | Cyclosporin A-treated Dendritic Cells may affect the outcome of organ transplantation by decreasing CD4+CD25+ regulatory T cell proliferation | |
