| dc.creator | Neri,Tommaso | |
| dc.creator | Conti,Ilaria | |
| dc.creator | Cerri,Chiara | |
| dc.creator | Tavanti,Laura | |
| dc.creator | Paggiaro,Pierluigi | |
| dc.creator | Celi,Alessandro | |
| dc.date | 2010-01-01 | |
| dc.date.accessioned | 2019-05-02T21:21:59Z | |
| dc.date.available | 2019-05-02T21:21:59Z | |
| dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000400012 | |
| dc.identifier.uri | http://revistaschilenas.uchile.cl/handle/2250/82100 | |
| dc.description | Nitric oxide (NO*) is a gaseous mediator synthesized by Nitric oxide sinthases. NO* is involved in the modulation of inflammation, but its role in airway inflammation remains controversial. We investigated the role of NO* in the synthesis of the chemok Nes Interleukin-8 and Monocyte Chemotactic Protein-1, and of Intercellular Adhesion Molecule-1 by human airway epithelial cells. normal human bronchial epithelial cells and the bronchial epithelial cell line BEAS-2B were used. Neterleukin-8 (IL-8) and Monocyte Chemotactic Protein-1 (MCP-1) secretion and Intercellular Adhesion Molecule-1 (ICAM-1) expression were measured by ELISA. mRNA was assessed by semiquantitative RTI-PCR. Neterleukin-8 secretion was significantly reduced after 24h incubation with the NO* donor, sodium nitroprusside. The effect was dose-dependent. Similar results were obta Ned with S-Nitroso-N-D,L-penicillam Ne and S-Nitroso-L-glutathione. Inhibition of endogenous NO* with the Nitric oxide synthase inhibitor N-Nitro-L-arg N Ne-methyl-esther caused an increase in IL-8 secretion by lypopolisaccharide- and cytok Ne-stimulated BEAS-2B cells. Sodium nitroprusside also caused a reduction in Monocyte Chemotactic Protein-1 secretion by both cell types. In contrast, Intercellular Adhesion Molecule-1 expression was upregulated by sodium NItroprusside. RTI-PCR results indícate that the modulation of protein levels was paralleled by modification in mRNA levels. NO* has divergent effects on the synthesis of different inflammatory mediators in human bronchial epithelial cells. | |
| dc.format | text/html | |
| dc.language | en | |
| dc.publisher | Sociedad de Biología de Chile | |
| dc.relation | 10.4067/S0716-97602010000400012 | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Biological Research v.43 n.4 2010 | |
| dc.subject | Adhesion molecules | |
| dc.subject | Airway inflammation | |
| dc.subject | Alveolar Epithelium | |
| dc.subject | Chemok Nes | |
| dc.subject | Nitric Oxide | |
| dc.title | Divergent effects of Nitric oxide on airway epithelial cell activation | |
