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dc.creatorHernandez,Pedro P
dc.creatorUndurraga,Cristian
dc.creatorGallardo,Viviana E
dc.creatorMackenzie,Natalia
dc.creatorAllende,Miguel L
dc.creatorReyes,Ariel E
dc.date2011-01-01
dc.date.accessioned2019-05-02T21:21:59Z
dc.date.available2019-05-02T21:21:59Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000100002
dc.identifier.urihttp://revistaschilenas.uchile.cl/handle/2250/82105
dc.descriptionCopper is an essential ion that forms part of the active sites of many proteins. At the same time, an excess of this metal produces free radicals that are toxic for cells and organisms. Fish have been used extensively to study the effects of metals, including copper, present in food or the environment. It has been shown that different metals induce different adaptive responses in adult fish. However, until now, scant information has been available about the responses that are induced by waterborne copper during early life stages of fish. Here, acute toxicity tests and LC50 curves have been generated for zebrafish larvae exposed to dissolved copper sulphate at different concentrations and for different treatment times. We determined that the larvae incorporate and accumulate copper present in the medium in a concentration-dependent manner, resulting in changes in gene expression. Using a transgenic fish line that expresses enhanced green fluorescent protein (EGFP) under the hsp70 promoter, we monitored tissue-specific stress responses to waterborne copper by following expression of the reporter. Furthermore, TUNEL assays revealed which tissues are more susceptible to cell death after exposure to copper. Our results establish a framework for the analysis of whole-organism management of excess external copper in developing aquatic animals.
dc.formattext/html
dc.languageen
dc.publisherSociedad de Biología de Chile
dc.relation10.4067/S0716-97602011000100002
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceBiological Research v.44 n.1 2011
dc.subjectacute exposure
dc.subjectapoptosis
dc.subjectcopper
dc.subjectearly life stage
dc.subjectheat shock
dc.subjectprotein-70
dc.subjectzebrafish
dc.titleSublethal concentrations of waterborne copper induce cellular stress and cell death in zebrafish embryos and larvae


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