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dc.creatorCohen,Débora J
dc.creatorMaldera,Julieta A
dc.creatorWeigel Muñoz,Mariana
dc.creatorErnesto,Juan I
dc.creatorVasen,Gustavo
dc.creatorCuasnicu,Patricia S
dc.date2011-01-01
dc.date.accessioned2019-05-02T21:22:00Z
dc.date.available2019-05-02T21:22:00Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000200004
dc.identifier.urihttp://revistaschilenas.uchile.cl/handle/2250/82120
dc.descriptionEpididymal protein CRISPI is a member of the CRISP (Cysteine-RIch Secretory proteins) family and is involved in sperm-egg fusion through its interaction with complementary sites on the egg surface. Results from our laboratory have shown that this binding ability resides in a 12-amino-acid region corresponding to a highly conserved motif of the CRISP family, named Signature 2 (S2). In addition to this, our results revealed that CRISP1 could also be involved in the previous step of sperm binding to the zona pellucida, identifying a novel role for this protein in fertilization. As another approach to elucidate the participation of CRISP1 in fertilization, a mouse line containing a targeted disruption of CRISP1 was generated. Although CRISP1-deficient mice exhibited normal fertility, CRISP1-defficient sperm presented a decreased level of protein tyrosine phosphorylation during capacitation, and an impaired ability to fertilize both zona-intact and zona-free eggs in vitro, confirming the proposed roles for the protein in fertilization. Evidence obtained in our laboratory indicated that testicular CRISP2 would also be involved in sperm-egg fusion. Competition assays between CRISP1 and CRISP2, as well as the comparison of their corresponding S2 regions, suggest that both proteins bind to common complementary sites in the egg. Together, these results suggest a functional cooperation between CRISP1 and CRISP2 to ensure the success of fertilization.
dc.formattext/html
dc.languageen
dc.publisherSociedad de Biología de Chile
dc.relation10.4067/S0716-97602011000200004
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceBiological Research v.44 n.2 2011
dc.subjectsperm
dc.subjectgamete fusion
dc.subjectCRISP
dc.subjectegg
dc.subjectfertilization
dc.titleCysteine-Rich Secretory Proteins (CRISP) and their role in mammalian fertilization


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