Rapid in vitro plant regeneration from leaf explants of Launaea sarmentosa (Willd.) Sch. Bip. ex Kuntze

dc.creator	Mahesh,Ayyavu
dc.creator	Thangadurai,Devarajan
dc.creator	Melchias,Gabriel
dc.date	2012-01-01
dc.date.accessioned	2019-05-02T21:22:05Z
dc.date.available	2019-05-02T21:22:05Z
dc.identifier	https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000200004
dc.identifier.uri	http://revistaschilenas.uchile.cl/handle/2250/82189
dc.description	An efficient protocol for organogenesis through leaves has been established for Launaea sarmentosa (Willd.) Sch. Bip. ex Kuntze, a highly valuable medicinal plant. The leaf explants produced microshoots on MS basal medium when fortified with cytokinins and auxins. A combination of 6-benzylaminopurine (BAP) at 0.5mg/l and naphthaleneacetic acid (NAA) at 0.2mg/l resulted in the induction of high frequency microshoots in 30 days. The microshoots were successfully subcultured for shoot elongation and eventually for rooting on MS medium supplemented with indole-3-butyric acid (IBA) at 0.5mg/l. The regenerated plantlets were hardened under greenhouse conditions and transferred to garden, resulting in a 90% survival rate.
dc.format	text/html
dc.language	en
dc.publisher	Sociedad de Biología de Chile
dc.relation	10.4067/S0716-97602012000200004
dc.rights	info:eu-repo/semantics/openAccess
dc.source	Biological Research v.45 n.2 2012
dc.subject	Asteraceae
dc.subject	Launaea sarmentosa
dc.subject	Leaf explant
dc.subject	Organogenesis
dc.title	Rapid in vitro plant regeneration from leaf explants of Launaea sarmentosa (Willd.) Sch. Bip. ex Kuntze