Show simple item record

dc.creatorMa,Jiao
dc.creatorYu,Dan
dc.creatorTong,Yu
dc.creatorMao,Meng
dc.date2012-01-01
dc.date.accessioned2019-05-02T21:22:08Z
dc.date.available2019-05-02T21:22:08Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000400005
dc.identifier.urihttp://revistaschilenas.uchile.cl/handle/2250/82245
dc.descriptionHypoxia-ischemia (HI) occurring in immature brains stimulates the expression of tissue-type plasminogen activator (tPA). Neuroserpin is a selected inhibitor of tPA in the central nerves system. However, the role that neuroserpin plays and the possible mechanisms involved during neonatal HI are poorly defined. In this study, an oxygen-glucose deprivation and reoxygenation (OGD/R) model was generated with cultured rat cortical neurons mimicking neonatal HI injury ex vivo, and an acute neuronal excitatory injury was induced by exposure to a high concentration of N-methyl-D-aspartic acid (NMDA). Cells received either neuroserpin or MK-801, an antagonist of the NMDA receptor, during OGD/R, and were incubated with or without neuroserpin after NMDA exposure. Cell viability and morphology were detected by a Cell Counting Kit-8 and immunohistochemical staining, respectively. TPA expression and activity were also assessed. We found that MK-801 alleviated injuries induced by OGD/R, suggesting an excitatory damage involvement. Neuroserpin provided a dose-dependent neuroprotective effect in both OGD/R and acute excitatory injuries by inhibiting the activity of tPA, without affecting neuronal tPA expression. Neuroserpin protected neurons against OGD/R even after a delayed administration of 3h. Collectively, our data indicate that neuroserpin protects neurons against OGD/R. mainly by inhibiting tPA-mediated acute neuronal excitotoxicity.
dc.formattext/html
dc.languageen
dc.publisherSociedad de Biología de Chile
dc.relation10.4067/S0716-97602012000400005
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceBiological Research v.45 n.4 2012
dc.subjectexcitotoxicity
dc.subjecthypoxia-ischemia
dc.subjectneuroserpin
dc.subjectN-methyl-D-aspartic
dc.subjecttissue-type plasminogen activator
dc.titleEffect of neuroserpin in a neonatal hypoxic-ischemic injury model ex vivo


This item appears in the following Collection(s)

Show simple item record