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dc.creatorMoresco,Rafaela M
dc.creatorManiglia,Thiago C
dc.creatorDe Oliveira,Classius
dc.creatorMargarido,Vladimir P
dc.date2013-01-01
dc.date.accessioned2019-05-02T21:22:10Z
dc.date.available2019-05-02T21:22:10Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000100008
dc.identifier.urihttp://revistaschilenas.uchile.cl/handle/2250/82272
dc.descriptionThe greatest diversity of anurans in the world is in Brazil and one of the major challenges is to reconcile the accelerated economic development with strategies that aim to maintain this diversity in forest fragments, often representing ESUs of some biomes. This study aimed to obtain data that will support conservation projects through the pioneering use of ISSR analysis in Neotropical anurans, estimating the intra- and interpopulation genetic diversity of four populations of P. cuvieri (Paraná and São Paulo regions). Of the 65 loci scored 58 were polymorphic, with 0.797 intrapopulation variation and 0.203 interpopulation variation. The index of interpopulation genetic differentiation (Fst) proved to be high among the population of Marmeleiro-PR and the three populations of SP (Fst > 0.288); genetic dissimilarity was related to the geographical distance. The ISSR proved to be efficient and useful molecular markers in comparison with other markers most widely used for preliminary diagnosis of genetic diversity in populations of amphibians, and could be applied as a tool for future conservation projects, since they could identify potential ESUs and influence decisions on the preservation of fragments.
dc.formattext/html
dc.languageen
dc.publisherSociedad de Biología de Chile
dc.relation10.4067/S0716-97602013000100008
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceBiological Research v.46 n.1 2013
dc.subjectESU
dc.subjectGene flow
dc.subjectGenetic structure
dc.subjectMolecular marker
dc.titleThe pioneering use of ISSR (Inter Simple Sequence Repeat) in Neotropical anurans: preliminary assessment of genetic diversity in populations of Physalaemus cuvieri (Amphibia, Leiuperidae)


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