| dc.creator | Vicuña,Lucas | |
| dc.creator | Pardo,Evelyn | |
| dc.creator | Curkovic,Cristóbal | |
| dc.creator | Doger,Remziye | |
| dc.creator | Oyanadel,Claudia | |
| dc.creator | Metz,Claudia | |
| dc.creator | Massardo,Loreto | |
| dc.creator | González,Alfonso | |
| dc.creator | Soza,Andrea | |
| dc.date | 2013-01-01 | |
| dc.date.accessioned | 2019-05-02T21:22:13Z | |
| dc.date.available | 2019-05-02T21:22:13Z | |
| dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000300008 | |
| dc.identifier.uri | http://revistaschilenas.uchile.cl/handle/2250/82327 | |
| dc.description | Galectin-8 belongs to a family of mammalian lectins that recognize glycoconjugates present on different cell surface components and modulate a variety of cellular processes. A role of Gal-8 in the immune system has been proposed based on its effects in immune cells, including T and B lymphocytes, as well as the presence of anti-Gal-8 autoantibodies in the prototypic autoimmune disease systemic lupus erythematosus (SLE). We have previously described that Gal-8 induces apoptosis in activated T cells interacting with certain β1 integrins and this effect is counteracted by the anti-Gal-8 autoantibodies. Given that Gal-8 can potentially interact with several glycoproteins, here we analyzed the β2 integrin Lymphocyte Function-Associated Antigen-1 (LFA-1), which is involved in leukocyte cell adhesion and immunological synapses. We show by GST-pull down assays that Gal-8 interacts with LFA-1 and this interaction is inhibited by anti-Gal-8 autoantibodies isolated from SLE patients. In cell adhesion assays, Gal-8 precluded the interaction of LFA-1 with its ligand Intracellular Adhesion Molecule-1 (ICAM-1). These results suggest that Gal-8 can exert immunosuppressive action not only by inducing apoptosis in activated T cells but also by negatively modulating the crucial function of LFA-1 in the immune system, while function-blocking autoantibodies counteract these effects. | |
| dc.format | text/html | |
| dc.language | en | |
| dc.publisher | Sociedad de Biología de Chile | |
| dc.relation | 10.4067/S0716-97602013000300008 | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Biological Research v.46 n.3 2013 | |
| dc.subject | Galectin-8 | |
| dc.subject | LFA-1 | |
| dc.subject | ICAM-1 | |
| dc.subject | SLE | |
| dc.subject | autoantibodies | |
| dc.title | Galectin-8 binds to LFA-1, blocks its interaction with ICAM-1 and is counteracted by anti-Gal-8 autoantibodies isolated from lupus patients | |
