| dc.creator | Bae,Seunghee | |
| dc.creator | Jung,Younjung | |
| dc.creator | Choi,Yeong Min | |
| dc.creator | Li,Shunhua | |
| dc.date | 2015-01-01 | |
| dc.date.accessioned | 2019-05-02T21:22:25Z | |
| dc.date.available | 2019-05-02T21:22:25Z | |
| dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100009 | |
| dc.identifier.uri | http://revistaschilenas.uchile.cl/handle/2250/82489 | |
| dc.description | BACKGROUND: Various health benefits have been attributed to Er-Miao-San (EMS), a traditional Chinese herbal formulation that contains equal amounts of cortex phellodendri (Phellodendron amurense Ruprecht) and rhizoma atractylodis (Atractylodes lancea D.C). However, its effect on the anti-inflammatory activity in human dermal fibroblasts (HDFs) and the mechanism underlying this effect are unknown. RESULTS: This study investigated the effects of EMS on TNF-α-induced MMP-1 expression in HDFs. Our data show that EMS inhibited TNF-α-induced MMP-1 expression in a concentration-dependent manner. Interestingly, EMS maintained IkB content without inhibiting the phosphorylation of MAPKs, which are well-established upstream kinases of NF-kB. Moreover, EMS reduced the level of nuclear p65 protein in HDFs. Luciferase assay revealed that EMS inhibits the transcriptional activity of NF-kBbystabilizing IkB. Our results show that EMS exerts its anti-inflammatory effect by inhibiting NF-kB-regulated genes such as ΙL-1β and IL-8. Moreover, EMS effectively inhibited TNF-α-induced expression of MMP-1 via the NF-kBpathway. CONCLUSIONS: Taken together, our data suggest that EMS could potentially be used as an anti-inflammatory and anti-aging treatment. | |
| dc.format | text/html | |
| dc.language | en | |
| dc.publisher | Sociedad de Biología de Chile | |
| dc.relation | 10.1186/0717-6287-48-8 | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Biological Research v.48 2015 | |
| dc.subject | Er-Miao-San | |
| dc.subject | MMP1 | |
| dc.subject | Human dermal fibroblasts | |
| dc.subject | TNF-α | |
| dc.subject | NF-kB | |
| dc.title | Effects of Er-Miao-San extracts on TNF-alpha-indueed MMP-1 expression in human dermal fibroblasts | |
