| dc.creator | Pan,Zeya | |
| dc.creator | Pan,Hao | |
| dc.creator | Zhang,Jin | |
| dc.creator | Yang,Yun | |
| dc.creator | Liu,Hui | |
| dc.creator | Yang,Yuan | |
| dc.creator | Huang,Gang | |
| dc.creator | Ni,Junsheng | |
| dc.creator | Huang,Jian | |
| dc.creator | Zhou,Weiping | |
| dc.date | 2015-01-01 | |
| dc.date.accessioned | 2019-05-02T21:22:25Z | |
| dc.date.available | 2019-05-02T21:22:25Z | |
| dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100018 | |
| dc.identifier.uri | http://revistaschilenas.uchile.cl/handle/2250/82505 | |
| dc.description | BACKGROUND: Ubiquitin Specific Peptidase 39 (USP39) is a 65 kDa SR-related protein involved in RNA splicing. Previous studies showed that USP39 is related with tumorigenesis of human breast cancer cells. RESULTS: In the present study, we investigated the functions of USP39 in human hepatocellular carcinoma (HCC) cell line SMMC-7721. We knocked down the expression of USP39 through lentivirus mediated RNA interference. The results of qRT-PCR and western blotting assay showed that both the mRNA and protein levels were suppressed efficiently after USP39 specific shRNA was delivered into SMMC-7721 cells. Cell growth was significantly inhibited as determined by MTT assay. Crystal violet staining indicated that colony numbers and sizes were both reduced after knock-down of USP39. Furthermore, suppression of USP39 arrested cell cycle progression at G2/M phase in SMMC-7721cells. In addition, Annexin V showed that downregulation of USP39 significantly increased the population of apoptotic cells. CONCLUSIONS: All our results suggest that USP39 is important for HCC cell proliferation and is a potential target for molecular therapy of HCC. | |
| dc.format | text/html | |
| dc.language | en | |
| dc.publisher | Sociedad de Biología de Chile | |
| dc.relation | 10.1186/s40659-015-0006-y | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Biological Research v.48 2015 | |
| dc.subject | Ubiquitin Specific Peptidase 39 | |
| dc.subject | Lentivirus | |
| dc.subject | Human hepatocellular carcinoma | |
| dc.subject | Cell proliferation | |
| dc.subject | Cell cycle | |
| dc.title | Lentivirus mediated silencing of Ubiquitin Specific Peptidase 39 inhibits cell proliferation of human hepatocellular carcinoma cells in vitro | |
