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Integrin-β1, not integrin-β5, mediates osteoblastic differentiation and ECM formation promoted by mechanical tensile strain

dc.creatorZeng,Qiangcheng
dc.creatorGuo,Yong
dc.creatorLiu,Yongming
dc.creatorLi,Ruixin
dc.creatorZhang,Xinchang
dc.creatorLiu,Lu
dc.creatorWang,Yang
dc.creatorZhang,Xizheng
dc.creatorZou,Xianqiong
dc.date2015-01-01
dc.date.accessioned2019-05-02T21:22:26Z
dc.date.available2019-05-02T21:22:26Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100025
dc.identifier.urihttp://revistaschilenas.uchile.cl/handle/2250/82516
dc.descriptionBACKGROUND: Mechanical strain plays a great role in growth and differentiation of osteoblast. A previous study indicated that integrin-β (β1, β5) mediated osteoblast proliferation promoted by mechanical tensile strain. However, the involvement of integrin-β in osteoblastic differentiation and extracellular matrix (ECM) formation induced by mechanical tensile strain, remains unclear. RESULTS: After transfection with integrin-β1 siRNA or integrin-β5 siRNA, mouse MC3T3-E1 preosteoblasts were cultured in cell culture dishes and stimulated with mechanical tensile strain of 2500 microstrain (µε) at 0.5 Hz applied once a day for 1 h over 3 or 5 consecutive days. The cyclic tensile strain promoted osteoblastic differentiation of MC3T3-E1 cells. Transfection with integrin-β1 siRNA attenuated the osteoblastic diffenentiation induced by the tensile strain. By contrast, transfection with integrin-β5 siRNA had little effect on the osteoblastic differentiation induced by thestrain. At thesametime, theresultofECM formation promoted by the strain, was similar to the osteoblastic differentiation. CONCLUSION: Integrin-β1 mediates osteoblast differentiation and osteoblastic ECM formation promoted by cyclic tensile strain, and integrin-β5 is not involved in the osteoblasts response to the tensile strain.
dc.formattext/html
dc.languageen
dc.publisherSociedad de Biología de Chile
dc.relation10.1186/s40659-015-0014-y
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceBiological Research v.48 2015
dc.subjectTensile strain
dc.subjectOsteoblast
dc.subjectExtracellular matrix
dc.subjectIntegrin
dc.subjectsiRNA
dc.titleIntegrin-β1, not integrin-β5, mediates osteoblastic differentiation and ECM formation promoted by mechanical tensile strain


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