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dc.creatorMatsumoto,Akiko
dc.creatorArisaka,Hirofumi
dc.creatorHosokawa,Yuki
dc.creatorSakuraba,Shigeki
dc.creatorSugita,Takeo
dc.creatorUmezawa,Nobuo
dc.creatorKaku,Yuki
dc.creatorYoshida,Kazu-ichi
dc.creatorKuwana,Shun-ichi
dc.date2015-01-01
dc.date.accessioned2019-05-02T21:22:28Z
dc.date.available2019-05-02T21:22:28Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100036
dc.identifier.urihttp://revistaschilenas.uchile.cl/handle/2250/82547
dc.descriptionBACKGROUND: The antiepileptic drugs carbamazepine and gabapentin are effective in treating neuropathic pain and trigeminal neuralgia. In the present study, to analyze the effects of carbamazepine and gabapentin on neuronal excitation in the spinal trigeminal subnucleus caudalis (Sp5c) in the medulla oblongata, we recorded temporal changes in nociceptive afferent activity in the Sp5c of trigeminal nerve-attached brainstem slices of neonatal rats using a voltage-sensitive dye imaging technique. RESULTS: Electrical stimulation of the trigeminal nerve rootlet evoked changes in the fluorescence intensity of dye in the Sp5c. The optical signals were composed of two phases, a fast component with a sharp peak followed by a long-lasting component with a period of more than 500 ms. This evoked excitation was not influenced by administration of carbamazepine (10, 100 and 1,000 μΜ) or gabapentin (1 and 10 μΜ), but was increased by administration of 100 μΜ gabapentin. This evoked excitation was increased further in low Mg²+ (0.8 mM) conditions, and this effect of low Mg²+ concentration was antagonized by 30 μM DL-2-amino-5-phosphonopentanoic acid (AP5), a N-methyl-D-as-partate (NMDA) receptor blocker. The increased excitation in low Mg²+ conditions was also antagonized by carbamazepine (1,000 μM) and gabapentin (100 μM). CONCLUSION: Carbamazepine and gabapentin did not decrease electrically evoked excitation in the Sp5c in control conditions. Further excitation in low Mg²+ conditions was antagonized by the NMDA receptor blocker AP5. Carbamazepine and gabapentin had similar effects to AP5 on evoked excitation in the Sp5c in low Mg²+ conditions. Thus, we concluded that carbamazepine and gabapentin may act by blocking NMDA receptors in the Sp5c, which contributes to its anti-hypersensitivity in neuropathic pain.
dc.formattext/html
dc.languageen
dc.publisherSociedad de Biología de Chile
dc.relation10.1186/S40659-015-0027-6
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceBiological Research v.48 2015
dc.subjectCarbamazepine
dc.subjectGabapentin
dc.subjectRat brainstem
dc.subjectSpinal trigeminal nucleus
dc.subjectVoltage-sensitive dye imaging
dc.titleEffect of carbamazepine and gabapentin on excitability in the trigeminal subnucleus caudalis of neonatal rats using a voltage-sensitive dye imaging technique


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