| dc.creator | Lee,Seahyoung | |
| dc.creator | Yun,Ina | |
| dc.creator | Ham,Onju | |
| dc.creator | Lee,Se-Yeon | |
| dc.creator | Lee,Chang Yeon | |
| dc.creator | Park,Jun-Hee | |
| dc.creator | Lee,Jiyun | |
| dc.creator | Seo,Hyang-Hee | |
| dc.creator | Choi,Eunhyun | |
| dc.creator | Hwang,Ki-Chul | |
| dc.date | 2015-01-01 | |
| dc.date.accessioned | 2019-05-02T21:22:29Z | |
| dc.date.available | 2019-05-02T21:22:29Z | |
| dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100045 | |
| dc.identifier.uri | http://revistaschilenas.uchile.cl/handle/2250/82564 | |
| dc.description | BACKGROUND: Low survival rate of transplanted cells compromises the efficacy of cell therapy. Hexokinase II (HKII) is known to have anti-apoptotic activity through its interaction with mitochondria. The objective was to identify miRNAs targeting HKII and investigate whether miRNA-mediated modulation of HKII could improve the survival of mesenchymal stem cells (MSCs) exposed to H2O2. The expression of HKII in MSCs exposed to H2O2 was evaluated, and HKII-targeting miRNA was screened based on miRNA-target prediction databases. The effect of H2O2 on the expression of the selected HKII-targeting miRNA was examined and the effect of modulation of the selected HKII-targeting miRNA using anti-miRNA on H2O2-induced apoptosis of MSC was evaluated. RESULTS: H2O2 (600 μΜ) induced cell death of MSCs and decreased mitochondrial HKII expression. We have identified miR-181a as a HKII-targeting miRNA and H2O2 increased the expression of miR-181a in MSCs. Delivery of anti-miR-181a, which neutralizes endogenous miR-181a, significantly attenuated H2O2-induced decrease of HKII expression and disruption of mitochondrial membrane potential, improving the survival of MSCs exposed to H2O2. CONCLUSIONS: These findings suggest that H2O2-induced up-regulation of miR-181a contributes to the cell death of MSCs by down-regulating HKII. Neutralizing miR-181a can be an effective way to prime MSCs for transplantation into ischemic tissues. | |
| dc.format | text/html | |
| dc.language | en | |
| dc.publisher | Sociedad de Biología de Chile | |
| dc.relation | 10.1186/S40659-015-0036-5 | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Biological Research v.48 2015 | |
| dc.subject | Hexokinase II | |
| dc.subject | miRNA-181a | |
| dc.subject | Cell death | |
| dc.subject | Mesenchymal stem cell | |
| dc.title | Suppression of miR-181 a attenuates H2O2-induced death of mesenchymal stem cells by maintaining hexokinase II expression | |
