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Colorectal cancer DNA methylation patterns from patients in Manaus, Brazil

dc.creatorAlmeida,Fabiana Greyce Oliveira
dc.creatorAquino,Priscila Ferreira de
dc.creatorSouza,Afonso Duarte Leäo de
dc.creatorSouza,Antonia Queiroz Lima de
dc.creatorVinhote,Sonia do Carmo
dc.creatorMac-Cormick,Thais Messias
dc.creatorSilva,Marcelo Soares da Mota
dc.creatorChalub,Sidney Raimundo Silva
dc.creatorFischer,Juliana de Saldanha da Gama
dc.creatorCarvalho,Paulo Costa
dc.creatorCarvalho,Maria da Gloria da Costa
dc.date2015-01-01
dc.date.accessioned2019-05-02T21:22:29Z
dc.date.available2019-05-02T21:22:29Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100050
dc.identifier.urihttp://revistaschilenas.uchile.cl/handle/2250/82572
dc.descriptionBACKGROUND: DNA methylation is commonly linked with the silencing of the gene expression for many tumor suppressor genes. As such, determining DNA methylation patterns should aid, in times to come, in the diagnosis and personal treatment for various types of cancers. Here, we analyzed the methylation pattern from five colorectal cancer patients from the Amazon state in Brazil for four tumor suppressor genes, viz.: DAPK, CDH1, CDKN2A, and TIMP2 by employing a polymerase chain reaction (PCR) specific to methylation. Efforts in the study of colorectal cancer are fundamental as it is the third most of highest incidence in the world. RESULTS: Tumor biopsies were methylated in 1/5 (20 %), 2/5 (40 %), 4/5 (80 %), and 4/5 (80 %) for CDH1, CDKN2A, DAPK, and TIMP2 genes, respectively. The margin biopsies were methylated in 3/7 (43 %), 2/7 (28 %), 7/7 (100 %), and 6/7 (86 %) for CDH1, CDKN2A, DAPK, and TIMP2, respectively. CONCLUSIONS: Our findings showed DAPK and TIMP2 to be methylated in most samples from both tumor tissues and adjacent non-neoplastic margins; thus presenting distinct methylation patterns. This emphasizes the importance of better understanding of the relation of these patterns with cancer in the context of different populations.
dc.formattext/html
dc.languageen
dc.publisherSociedad de Biología de Chile
dc.relation10.1186/S40659-015-0042-7
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceBiological Research v.48 2015
dc.subjectMethylation
dc.subjectColorectal cancer
dc.subjectAmazon
dc.subjectEpigenetic
dc.titleColorectal cancer DNA methylation patterns from patients in Manaus, Brazil


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