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dc.creatorOrozco-Ibarra,Marisol
dc.creatorMuñoz-Sánchez,Jorge
dc.creatorZavala-Medina,Martín E
dc.creatorPineda,Benjamín
dc.creatorMagaña-Maldonado,Roxana
dc.creatorVázquez-Contreras,Edgar
dc.creatorMaldonado,Perla D
dc.creatorPedraza-Chaverri,José
dc.creatorChánez-Cárdenas,María Elena
dc.date2016-01-01
dc.date.accessioned2019-05-02T21:22:32Z
dc.date.available2019-05-02T21:22:32Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100007
dc.identifier.urihttp://revistaschilenas.uchile.cl/handle/2250/82612
dc.descriptionBACKGROUND: Aged garlic extract (AGE) and its main constituent S-allylcysteine (SAC) are natural antioxidants with protective effects against cerebral ischemia or cancer, events that involve hypoxia stress. Cobalt chloride (CoCl2) has been used to mimic hypoxic conditions through the stabilization of the α subunit of hypoxia inducible factor (HIF-Ια) and up-regulation of HIF-1a-dependent genes as well as activation of hypoxic conditions such as reactive oxygen species (ROS) generation, loss of mitochondrial membrane potential and apoptosis. The present study was designed to assess the effect of AGE and SAC on the CoCl2-chemical hypoxia model in PC12 cells RESULTS: We found that CoCl2 induced the stabilization of HIF-1a and its nuclear localization. CoCl2 produced ROS and apoptotic cell death that depended on hypoxia extent. The treatment with AGE and SAC decreased ROS and protected against CoCl2-induced apoptotic cell death which depended on the CoCl2 concentration and incubation time. SAC or AGE decreased the number of cells in the early and late stages of apoptosis. Interestingly, this protective effect was associated with attenuation in HIF-1a stabilization, activity not previously reported for AGE and SAC CONCLUSIONS: Obtained results show that AGE and SAC decreased apoptotic CoCl2-induced cell death. This protection occurs by affecting the activity of HIF-1a and supports the use of these natural compounds as a therapeutic alternative for hypoxic conditions
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dc.languageen
dc.publisherSociedad de Biología de Chile
dc.relation10.1186/s40659-016-0067-6
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceBiological Research v.49 2016
dc.subjectAged garlic extract
dc.subjectChemical hypoxia
dc.subjectCobalt chloride
dc.subjectHypoxia-inducible factor 1
dc.subjectPC12 cells
dc.subjectS-allylcysteine
dc.titleAged garlic extract and S-allylcysteine prevent apoptotic cell death in a chemical hypoxia model


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