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dc.creatorMarthandan,Shiva
dc.creatorMenzel,Uwe
dc.creatorPriebe,Steffen
dc.creatorGroth,Marco
dc.creatorGuthke,Reinhard
dc.creatorPlatzer,Matthias
dc.creatorHemmerich,Peter
dc.creatorKaether,Christoph
dc.creatorDiekmann,Stephan
dc.date2016-01-01
dc.date.accessioned2019-05-02T21:22:35Z
dc.date.available2019-05-02T21:22:35Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100034
dc.identifier.urihttp://revistaschilenas.uchile.cl/handle/2250/82659
dc.descriptionBACKGROUND: Cellular senescence is induced either internally, for example by replication exhaustion and cell division, or externally, for example by irradiation. In both cases, cellular damages accumulate which, if not successfully repaired, can result in senescence induction. Recently, we determined the transcriptional changes combined with the transition into replicative senescence in primary human fibroblast strains. Here, by γ-irradiation we induced premature cellular senescence in the fibroblast cell strains (HFF and MRC-5) and determined the corresponding transcriptional changes by high-throughput RNA sequencing. RESULTS: Comparing the transcriptomes, we found a high degree of similarity in differential gene expression in replicative as well as in irradiation induced senescence for both cell strains suggesting, in each cell strain, a common cellular response to error accumulation. On the functional pathway level, "Cell cycle" was the only pathway commonly down-regulated in replicative and irradiation-induced senescence in both fibroblast strains, confirming the tight link between DNA repair and cell cycle regulation. However, "DNA repair" and "replication" pathways were down-regulated more strongly in fibroblasts undergoing replicative exhaustion. We also retrieved genes and pathways in each of the cell strains specific for irradiation induced senescence. CONCLUSION: We found the pathways associated with "DNA repair" and "replication" less stringently regulated in irradiation induced compared to replicative senescence. The strong regulation of these pathways in replicative senescence highlights the importance of replication errors for its induction.
dc.formattext/html
dc.languageen
dc.publisherSociedad de Biología de Chile
dc.relation10.1186/s40659-016-0095-2
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceBiological Research v.49 2016
dc.subjectSenescence
dc.subjectFibroblasts
dc.subjectγ-irradiation
dc.subjectAging
dc.subjectTranscriptome analysis
dc.subjectDNA repair
dc.titleConserved genes and pathways in primary human fibroblast strains undergoing replicative and radiation induced senescence


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