| dc.creator | Han,Xu | |
| dc.creator | Wang,Lingling | |
| dc.creator | Ning,Yu | |
| dc.creator | Li,Shuang | |
| dc.creator | Wang,Zhenjun | |
| dc.date | 2016-01-01 | |
| dc.date.accessioned | 2019-05-02T21:22:36Z | |
| dc.date.available | 2019-05-02T21:22:36Z | |
| dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100036 | |
| dc.identifier.uri | http://revistaschilenas.uchile.cl/handle/2250/82662 | |
| dc.description | BACKGROUND AND OBJECTIVE: Long non-coding RNAs can regulate tumorigenesis of various cancers. Dys-regulation of lncRNA-AFAP1-AS1 has not been studied in colorectal carcinoma (CRC). This study was to examine the function involvement of AFAP1-AS1 in tumor growth and metastasis of CRC. METHODS: Relative expression of AFAP1-AS1 in CRC tissues and CRC cells lines was determined using quantitative real-time PCR (qRT-PCR). Functional involvement of AFAP1-AS1 in tumor proliferation and metastasis was evaluated in AFAP1-AS1-specific siRNA-treated CRC cells and in CRC cell xenograft. Expression of epithelial-mesenchymal transition (EMT)-related gene expression was determined using western blot. RESULTS: Relative expression of AFAP1-AS1 was significantly elevated in CRC tissues and CRC HCT116 and SW480 cell lines. AFAP1-AS1 knock-down suppressed SW480 cell proliferation, colony formation, migration and invasion. Also AFAP1-AS1 knock-down inhibited tumor metastasis-associated genes expression in terms of EMT. This carcinostatic action by AFAP1-AS1 knock-down was further confirmed by suppression of tumor formation and hepatic metastasis of CRC cells in nude mice. CONCLUSION: lncRNA-AFAP1-AS1 knock-down exhibits antitumor effect on colorectal carcinoma in respects of suppression of cell proliferation and metastasis of cancer cells. | |
| dc.format | text/html | |
| dc.language | en | |
| dc.publisher | Sociedad de Biología de Chile | |
| dc.relation | 10.1186/s40659-016-0094-3 | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Biological Research v.49 2016 | |
| dc.subject | AFAP1-AS1 | |
| dc.subject | Colorectal cancer | |
| dc.subject | Tumor metastasis | |
| dc.subject | Epithelial-mesenchymal transition (EMT) | |
| dc.title | Long non-coding RNA AFAP1-AS1 facilitates tumor growth and promotes metastasis in colorectal cancer | |
