| dc.creator | Cheng,Jin | |
| dc.creator | Yi,Zhang | |
| dc.creator | Jiping,Li | |
| dc.date | 2016-01-01 | |
| dc.date.accessioned | 2019-05-02T21:22:36Z | |
| dc.date.available | 2019-05-02T21:22:36Z | |
| dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100040 | |
| dc.identifier.uri | http://revistaschilenas.uchile.cl/handle/2250/82669 | |
| dc.description | BACKGROUND: Accumulating evidence has confirmed that miR-196a plays a critical role in tumorigenesis and tumor progression in a variety of cancers. It has been demonstrated that miR-196a is highly up-regulated in laryngeal cancer by miRNA profiling analysis. However, the functional mechanism of miR-196a in laryngeal cancer remains unclear. This study aims to explore the mechanism of miR-196a in laryngeal cancer METHODS: In the present study, we conducted qPCR analysis of miR-196a expression in human laryngeal cancer and showed that miR-196a was overexpressed in tumor-derived samples and laryngeal cancer cell lines compared with matched normal controls. Further functional analysis of miR-196a demonstrated that the inhibition of miR-196a could inhibit laryngeal cell-cycle progression and proliferation in vitro. Luciferase reporter assay and western blot confirmed that miR-196a directly targeted p27kip1. Moreover, in order to investigate whether miR-196a regulated cell growth in laryngeal cancer cells by targeting p27kip1, rescue studies were performed in laryngeal cancer cells RESULTS: Results showed that overexpression of p27kip1 rescue decreased cell proliferation caused by miR-196a inhibitors. A negative relation between miR-196a and p27kip1 expression in laryngeal cancer tissues were also noted by further analyses CONCLUSIONS: The present study showed that miR-196a was upregulated in laryngeal cancer and promoted cell proliferation by downregulating p27kip1 in laryngeal cancer. However, further studies are needed to verify this finding | |
| dc.format | text/html | |
| dc.language | en | |
| dc.publisher | Sociedad de Biología de Chile | |
| dc.relation | 10.1186/s40659-016-0100-9 | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Biological Research v.49 2016 | |
| dc.subject | miR-196a | |
| dc.subject | p27kp1 | |
| dc.subject | Laryngeal cancer | |
| dc.title | Upregulation of MiR-196a promotes cell proliferation by downregulating p27kip1 in laryngeal cancer | |
