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dc.creatorWang,Hui
dc.creatorLiu,Zheng
dc.creatorZhang,Wenxiu
dc.creatorYuan,Ziao
dc.creatorYuan,Hongyi
dc.creatorLiu,Xueting
dc.creatorYang,Chunwen
dc.creatorGuan,Weijun
dc.date2016-01-01
dc.date.accessioned2019-05-02T21:22:36Z
dc.date.available2019-05-02T21:22:36Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100042
dc.identifier.urihttp://revistaschilenas.uchile.cl/handle/2250/82673
dc.descriptionBACKGROUND: Heavy metals can cause great harm to Siberian tigers in the natural environment. Cadmium (Cd2+) is an environmental contaminant that affects multiple cellular processes, including cell proliferation, differentiation, and survival. It has been shown to induce apoptosis in a variety of cell types and tissues. RESULTS: We investigated the apoptotic effects of Cd2+ on Siberian tiger fibroblasts in vitro. Our research revealed the typical signs of apoptosis after Cd²+ exposure. Apoptosis was dose- (0-4.8 μΜ) and duration-dependent (12-48 h), and proliferation was strongly inhibited. Cd²+ increased the activity of caspase-3, -8, and -9 and disrupted calcium homeostasis by causing oxidative stress and mitochondrial dysfunction. It also increased K+ efflux and altered the mRNA levels of Bax, Bcl-2, caspase-3, caspase-8, Fas, and p53. CONCLUSIONS: Our results suggest that Cd2+ triggers the apoptosis of Siberian tiger fibroblasts by disturbing intracellular homeostasis. These results will aid in our understanding of the effects of Cd2+ on Siberian tigers and in developing interventions to treat and prevent cadmium poisoning.
dc.formattext/html
dc.languageen
dc.publisherSociedad de Biología de Chile
dc.relation10.1186/s40659-016-0103-6
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceBiological Research v.49 2016
dc.subjectSiberian tiger
dc.subjectCadmium
dc.subjectApoptosis
dc.subjectFibroblast
dc.subjectCalcium
dc.titleCadmium-induced apoptosis of Siberian tiger fibroblasts via disrupted intracellular homeostasis


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