dc.creator | Wang,Hui | |
dc.creator | Liu,Zheng | |
dc.creator | Zhang,Wenxiu | |
dc.creator | Yuan,Ziao | |
dc.creator | Yuan,Hongyi | |
dc.creator | Liu,Xueting | |
dc.creator | Yang,Chunwen | |
dc.creator | Guan,Weijun | |
dc.date | 2016-01-01 | |
dc.date.accessioned | 2019-05-02T21:22:36Z | |
dc.date.available | 2019-05-02T21:22:36Z | |
dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100042 | |
dc.identifier.uri | http://revistaschilenas.uchile.cl/handle/2250/82673 | |
dc.description | BACKGROUND: Heavy metals can cause great harm to Siberian tigers in the natural environment. Cadmium (Cd2+) is an environmental contaminant that affects multiple cellular processes, including cell proliferation, differentiation, and survival. It has been shown to induce apoptosis in a variety of cell types and tissues. RESULTS: We investigated the apoptotic effects of Cd2+ on Siberian tiger fibroblasts in vitro. Our research revealed the typical signs of apoptosis after Cd²+ exposure. Apoptosis was dose- (0-4.8 μΜ) and duration-dependent (12-48 h), and proliferation was strongly inhibited. Cd²+ increased the activity of caspase-3, -8, and -9 and disrupted calcium homeostasis by causing oxidative stress and mitochondrial dysfunction. It also increased K+ efflux and altered the mRNA levels of Bax, Bcl-2, caspase-3, caspase-8, Fas, and p53. CONCLUSIONS: Our results suggest that Cd2+ triggers the apoptosis of Siberian tiger fibroblasts by disturbing intracellular homeostasis. These results will aid in our understanding of the effects of Cd2+ on Siberian tigers and in developing interventions to treat and prevent cadmium poisoning. | |
dc.format | text/html | |
dc.language | en | |
dc.publisher | Sociedad de Biología de Chile | |
dc.relation | 10.1186/s40659-016-0103-6 | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.source | Biological Research v.49 2016 | |
dc.subject | Siberian tiger | |
dc.subject | Cadmium | |
dc.subject | Apoptosis | |
dc.subject | Fibroblast | |
dc.subject | Calcium | |
dc.title | Cadmium-induced apoptosis of Siberian tiger fibroblasts via disrupted intracellular homeostasis | |