| dc.creator | González,Luis F. | |
| dc.creator | Henríquez-Belmar,Francisca | |
| dc.creator | Delgado-Acevedo,Claudia | |
| dc.creator | Cisternas-Olmedo,Marisol | |
| dc.creator | Arriagada,Gloria | |
| dc.creator | Sotomayor-Zárate,Ramón | |
| dc.creator | Murphy,Dennis L. | |
| dc.creator | Moya,Pablo R. | |
| dc.date | 2017-01-01 | |
| dc.date.accessioned | 2019-05-02T21:22:39Z | |
| dc.date.available | 2019-05-02T21:22:39Z | |
| dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100225 | |
| dc.identifier.uri | http://revistaschilenas.uchile.cl/handle/2250/82722 | |
| dc.description | Abstract Background Obsessive–compulsive disorder (OCD) is a severe neuropsychiatric condition affecting 1–3% of the worldwide population. OCD has a strong genetic component, and the SLC1A1 gene that encodes neuronal glutamate transporter EAAT3 is a strong candidate for this disorder. To evaluate the impact of reduced EAAT3 expression in vivo, we studied male EAAT3 heterozygous and wild-type littermate mice using a battery of behavioral paradigms relevant to anxiety (open field test, elevated plus maze) and compulsivity (marble burying), as well as locomotor activity induced by amphetamine. Using high-performance liquid chromatography, we also determined tissue neurotransmitter levels in cortex, striatum and thalamus—brain areas that are relevant to OCD. Results Compared to wild-type littermates, EAAT3 heterozygous male mice have unaltered baseline anxiety-like, compulsive-like behavior and locomotor activity. Administration of acute amphetamine (5 mg/kg intraperitoneally) increased locomotion with no differences across genotypes. Tissue levels of glutamate, GABA, dopamine and serotonin did not vary between EAAT3 heterozygous and wild-type mice. Conclusions Our results indicate that reduced EAAT3 expression does not impact neurotransmitter content in the corticostriatal circuit nor alter anxiety or compulsive-like behaviors. | |
| dc.format | text/html | |
| dc.language | en | |
| dc.publisher | Sociedad de Biología de Chile | |
| dc.relation | 10.1186/s40659-017-0138-3 | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Biological Research v.50 2017 | |
| dc.subject | EAAT3 | |
| dc.subject | SLC1A1 | |
| dc.subject | Neuronal glutamate transporter | |
| dc.subject | Obsessive–compulsive disorder | |
| dc.title | Neurochemical and behavioral characterization of neuronal glutamate transporter EAAT3 heterozygous mice | |
