| dc.creator | Du,Maotao | |
| dc.creator | Zhang,Zhong | |
| dc.creator | Gao,Tao | |
| dc.date | 2017-01-01 | |
| dc.date.accessioned | 2019-05-02T21:22:39Z | |
| dc.date.available | 2019-05-02T21:22:39Z | |
| dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100226 | |
| dc.identifier.uri | http://revistaschilenas.uchile.cl/handle/2250/82723 | |
| dc.description | Abstract Background Melanoma took top position among the lethal cancers and, despite there have been some great attempts made to increase the natural life of patients with metastatic disease, long-lasting and complete remissions are few. Piceatannol, owns the similar function as resveratrol, has been defined as an anti-cancer agent playing important role in inhibition of proliferation, migration and metastasis in various cancer. Thus, we aim to investigate the anti-cancer effect and mechanisms of piceatannol in melanoma cells. Methods Melanoma cell lines WM266-4 and A2058 were treated either with or without piceatannol. Cell viability and cell apoptosis were assessed by using MTT and Annexin V/PI assay, respectively. Cells were transfected with specific miRNA using Lipfectamine 2000. miRNA bingding ability to 3'-UTR region within specific gene was assed by firefly luciferase analysis. Gene and protein expression was eveluated by qRT-PCR and western blot analysis, respectively. Results Our study showed that piceatannol inhibited WM266-4 and A2058 cells growth and induced apoptosis. Totally, 16 differentially expressed miRNAs were screened out including 8 up-regulated and 8 down-regulated miRNAs. Expression level of miR-181a is significantly higher in piceatannol-treated cells than normal control and is lower in melanoma cancer tissues than its adjacent normal tissues. Bcl-2 is a target gene of miR-181a. Moreover, silencing of miR-181a reverses the decrease of cell viability induced by piceatannol in WM266-4 and A2058 cells. Taken together, present study uncovered the ability of piceatannol to repress melanoma cell growth and clarified the contribution of miR-181a in the anticancer role of piceatannol. Conclusion The present study proposes that piceatannol can be taken into account to be a hopeful anticancer agent for melanoma. | |
| dc.format | text/html | |
| dc.language | en | |
| dc.publisher | Sociedad de Biología de Chile | |
| dc.relation | 10.1186/s40659-017-0141-8 | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Biological Research v.50 2017 | |
| dc.subject | Piceatannol | |
| dc.subject | Apoptosis | |
| dc.subject | microRNA-181-a | |
| dc.subject | Melanoma cells | |
| dc.title | Piceatannol induced apoptosis through up-regulation of microRNA-181a in melanoma cells | |
