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dc.creatorAgrawal,Sweta
dc.creatorChaugule,Sachin
dc.creatorMore,Shashank
dc.creatorRane,Gargi
dc.creatorIndap,Madhavi
dc.date2017-01-01
dc.date.accessioned2019-05-02T21:22:39Z
dc.date.available2019-05-02T21:22:39Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100231
dc.identifier.urihttp://revistaschilenas.uchile.cl/handle/2250/82728
dc.descriptionAbstract Background: The marine environment is a rich source of bioactive natural products. Many of the marine bioactive compounds have been derived successfully from molluscs. Euchelus asper is a marine mollusc which is commonly found in the intertidal rocky regions of the Mumbai coast. The present study was focused on evaluating the anti-angiogenic and anti- proliferative activities of methanolic extract of Euchelus asper (EAME). Methods: The anti-angiogenic activity of EAME (50–800 μg/mL) was assessed by chick chorio-allantoic membrane (CAM) model wherein multiple parameters in the CAM blood vessels were analysed through morphometric and histo-logical investigations. In vitro testing of EAME (5–20 μg/mL) included its cytotoxicity against three different cancer cell lines, its effect on cell proliferation by wound healing assay as well as their relevant molecular mechanisms. Statistical analysis was carried out by two-tailed student's t test for two unpaired groups. Results: Analysis of CAM revealed that the extract is effective in reducing the branching points of the 1st order blood vessels or capillaries of CAM. Histological analysis of CAM showed significant decrease in capillary plexus and compartmentalization along with increase in mesodermal blood vessels, thus establishing its anti-angiogenicity. Further, EAME exhibited moderate but significant cytotoxicity against A549 non-small cell lung carcinoma cell line. We also demonstrated that the cytotoxicity of EAME in A549 was associated with its apoptotic activity by subG1 phase arrest. Lastly, EAME significantly reduced A549 proliferation by reducing the expression of Matrix metalloproteinase-2 (MMP-2) and Matrix metalloproteinase-9 (MMP-9). Conclusion: Overall, our study suggested that EAME has potential to inhibit tumour angiogenic and proliferative activity and may be a potential source for development of new anti-cancer pharmaceuticals.
dc.formattext/html
dc.languageen
dc.publisherSociedad de Biología de Chile
dc.relation10.1186/s40659-017-0147-2
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceBiological Research v.50 2017
dc.subjectMarine bioactives
dc.subjectEuchelus asper
dc.subjectMollusc
dc.subjectAnti-angiogenesis
dc.subjectAnti-proliferative
dc.subjectAnti-cancer
dc.subjectCAM
dc.subjectChorioallantoic membrane
dc.titleMethanolic extract of Euchelus asper exhibits in-ovo anti-angiogenic and in vitro anti-proliferative activities


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