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dc.creatorLi,Yu
dc.creatorZhang,Depeng
dc.creatorYu,Kaikai
dc.creatorHu,Yudong
dc.creatorWu,Qiong
dc.creatorQian,Feng
dc.creatorWang,Zishu
dc.date2018-01-01
dc.date.accessioned2019-05-02T21:22:42Z
dc.date.available2019-05-02T21:22:42Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0716-97602018000100210
dc.identifier.urihttp://revistaschilenas.uchile.cl/handle/2250/82749
dc.descriptionAbstract Background Gastric cancer occupies the fourth highest morbidity rate of cancers worldwide. Clinical therapies of gastric cancer remain limited because of uncertainty of mechanisms and shortness of effective medicine. Thus, new drug candidates for gastric cancer treatment is urgently needed. Results In this study, CMPD1 as a wildly used MK2 phosphorylation inhibitor was employed to find its impact on gastric cancer cell proliferation, apoptosis and cell cycle using colony formation assay and flow cytometry analysis. Along with its anti-proliferation effect on gastric cancer cell line MKN-45 and SGC7901, CMPD1 also induced massive apoptosis and significant G2/M phase arrest in a time-dependent and dose-dependent manner in MKN-45 cells respectively. Furthermore, Western blot confirmed that the expression of anti-apoptotic proteins Bcl-2 was decreased while BAX, cytochrome c release and cleaved PARP were increased. In addition, oncogene c-Myc was downregulated in response to CMPD1 treatment. Conclusions Our results demonstrated that CMPD1 has anti-tumor effect on human gastric cancer cell line MKN- 45 possibly via downregulating oncogene c-Myc expression and CMPD1 could be applied as a potential candidate for treating gastric malignancy. To the best of our knowledge, it is the first report of anti-tumor effect of CMPD-1 on human gastric cancer cells.
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dc.languageen
dc.publisherSociedad de Biología de Chile
dc.relation10.1186/s40659-018-0159-6
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceBiological Research v.51 2018
dc.subjectCMPD1
dc.subjectProliferation
dc.subjectApoptosis
dc.subjectCell cycle arrest
dc.subjectMKN-45
dc.titleCMPD1 inhibited human gastric cancer cell proliferation by inducing apoptosis and G2/M cell cycle arrest


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