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dc.creatorde la Riva,Gustavo A
dc.creatorGonzález-Cabrera,Joel
dc.creatorVázquez-Padrón,Roberto
dc.creatorAyra-Pardo,Camilo
dc.date1998-12-01
dc.date.accessioned2019-05-03T12:43:53Z
dc.date.available2019-05-03T12:43:53Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34581998000300002
dc.identifier.urihttp://revistaschilenas.uchile.cl/handle/2250/84623
dc.descriptionUpdated information of mechanisms for T-DNA transfer to plant cells by Agrobacterium tumefaciens is provided, focused on the role played by the different components of the virulence system. The general assessments for the establishment of efficient transformation protocols are discussed with an emphasis in the application of this methodology to monocotyledonous plants. Based on our own experience, we present the establishment of sugarcane transformation by A. tumefaciens as a model of application of this methodology to an important culture plant species, previously considered recalcitrant and inaccessible for this type of genetic manipulation.
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dc.languageen
dc.publisherPontificia Universidad Católica de Valparaíso
dc.relation10.4067/S0717-34581998000300002
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceElectronic Journal of Biotechnology v.1 n.3 1998
dc.titleAgrobacterium tumefaciens: a natural tool for plant transformation


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