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  • Electronic Journal of Biotechnology
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Solid-state fermentation of Bacillus thuringiensis tolworthi to control fall armyworm in maize

Author
Fontana Capalbo,Deise Maria

Valicente,Fernando Hercule

Oliveira Moraes,Iracema de

Pelizer,Lúcia Helena

Full text
https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582001000200009
Abstract
The well-known entomopathogenic bacterium Bacillus thuringiensis (Bt) produces a spore-crystal complex which is responsible for its biocide characteristic, and the bacterium can be obtained by fermentation, either in liquid or semi-solid substrates. This paper presents a successful way to achieve solid-state fermentation of active Bt var. tolworthi (Btt) against Spodoptera frugiperda (fall armyworm) in corn. More than 10(9) CFU/g were obtained using humidified rice as substrate maintained in polypropylene bags. This active complex (substrate plus spore-crystal of Bt) was prepared in order to obtain 2 x 10(6) spores/mL; the final suspension then sprayed via tractor on corn fields. On the treated plants, mortality of neonate larvae was 100% within two days of spraying, and all larvae were found dead on leaves. During one maize crop cycle, two applications were made, and up until 70 days after emergence it was not necessary to apply any other insecticide for fall armyworm control.
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Discipline
Artes, Arquitectura y UrbanismoCiencias Agrarias, Forestales y VeterinariasCiencias Exactas y NaturalesCiencias SocialesDerechoEconomía y AdministraciónFilosofía y HumanidadesIngenieríaMedicinaMultidisciplinarias
Institutions
Universidad de ChileUniversidad Católica de ChileUniversidad de Santiago de ChileUniversidad de ConcepciónUniversidad Austral de ChileUniversidad Católica de ValparaísoUniversidad del Bio BioUniversidad de ValparaísoUniversidad Católica del Nortemore

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