| dc.creator | Kopytko,Maria | |
| dc.creator | Chalela,Graciela | |
| dc.creator | Zauscher,Fernando | |
| dc.date | 2002-08-01 | |
| dc.date.accessioned | 2019-05-03T12:44:02Z | |
| dc.date.available | 2019-05-03T12:44:02Z | |
| dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582002000200013 | |
| dc.identifier.uri | http://revistaschilenas.uchile.cl/handle/2250/84717 | |
| dc.description | The purpose of this project was to evaluate the biodegradation of two commercial herbicides (Gramoxone and Matancha) by the bacteria Pseudomonas putida. Gramoxone s active ingredient is Paraquat (1,1 -dimethyl-4,4 -bipyridylium) and Matancha s is 2,4-D (2,4-dichlorophenoxiacetic acid). To carry out the biodegradation experiments, a factorial design 2³ was executed, where the independent variables were: herbicide concentration, nutrient concentration and the use or no use of activated charcoal (AC) in the same experiment with bacteria. Duplicate experiments were performed for a period of three days using 500 ml bioreactors. The best results obtained when no AC was used were: 47,29% degradation for Gramoxone (by UV/VIS) and 68.72% for Matancha (by HPLC). The treatments carried out with the use of AC for either of the two herbicides gave more than 95% degradation at 72 h. In fact, under these conditions most of the experiments showed at least a 90% removal at 24 h. It is clear that the dual combination of adsorption by AC and biological activity by the bacteria was very effective, in particular when at least 10% nutrients were added. The statistical study of the results showed that AC is a highly significant variable, and that there are significant interactions between the three variables used in the experimental design. The application of these experiments to wastewater (COD = 584.11) confirmed that it is possible to remove more than 90% of either of these herbicides in a 24 h period. | |
| dc.format | text/html | |
| dc.language | en | |
| dc.publisher | Pontificia Universidad Católica de Valparaíso | |
| dc.relation | 10.4067/S0717-34582002000200013 | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Electronic Journal of Biotechnology v.5 n.2 2002 | |
| dc.title | Biodegradation of two commercial herbicides (Gramoxone and Matancha) by the bacteria Pseudomonas putida | |
