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dc.creatorLakrod,Kamolnan
dc.creatorChaisrisook,Chulee
dc.creatorSkinner,Daniel Z
dc.date2003-08-01
dc.date.accessioned2019-05-03T12:44:04Z
dc.date.available2019-05-03T12:44:04Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582003000200007
dc.identifier.urihttp://revistaschilenas.uchile.cl/handle/2250/84746
dc.descriptionAlbino strain KB20M1 of Monascus purpureus was genetically transformed to hygromycin B resistance with cosmid pMOcosX, using biolistic bombardment. Conidia and mycelial fragments were used as the recipient material and rupture disk strengths of 6.2 and 9.3 MPa each yielded a transformed isolate from five bombardments. Southern analysis suggested a single copy of the cosmid had integrated into each of the transformants. Both of the independent transformants formed cleistothecia but ascospore formation was greatly reduced or absent, suggesting the integration and expression of the genes carried on pMOcosX interfered with fertilization and/or ascospore formation in this homothallic fungus.
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dc.languageen
dc.publisherPontificia Universidad Católica de Valparaíso
dc.relation10.4067/S0717-34582003000200007
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceElectronic Journal of Biotechnology v.6 n.2 2003
dc.titleTransformation of Monascus purpureus to hygromycin B resistance with cosmid pMOcosX reduces fertility


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