| dc.creator | Trujillo Toledo,Luis Enrique | |
| dc.creator | Gómez Riera,Raúl | |
| dc.creator | Banguela Castillo,Alexander | |
| dc.creator | Soto Romero,Melvis | |
| dc.creator | Arrieta Sosa,Juan Gabriel | |
| dc.creator | Hernández García,Lázaro | |
| dc.date | 2004-08-01 | |
| dc.date.accessioned | 2019-05-03T12:44:07Z | |
| dc.date.available | 2019-05-03T12:44:07Z | |
| dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582004000200005 | |
| dc.identifier.uri | http://revistaschilenas.uchile.cl/handle/2250/84777 | |
| dc.description | The influence of N-glycosylation on the kinetic and catalytical properties of a bacterial fructosyltransferase (LsdA) produced in Pichia pastoris was studied. The glycosylated enzyme behaved similarly to non-glycosylated LsdA when substrate specificity, fructo-oligosaccharide (FOS) production, sucrose hydrolysis or levan formation reactions were carried out under different experimental conditions. The kinetic parameters for native or yeast-expressed LsdA determined at 60ºC, condition for the highest hydrolytic activity, followed a conventional Michaelis-Menten kinetics. Synthase activity of this levansucrase increased in water-restricted environments by addition of salt or organic solvent to the reaction mixtures | |
| dc.format | text/html | |
| dc.language | en | |
| dc.publisher | Pontificia Universidad Católica de Valparaíso | |
| dc.relation | 10.4067/S0717-34582004000200005 | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Electronic Journal of Biotechnology v.7 n.2 2004 | |
| dc.title | Catalytical properties of N-glycosylated Gluconacetobacter diazotrophicus levansucrase produced in yeast | |
