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dc.creatorVargas,Teresa E.
dc.creatorMejías,Alexander
dc.creatorOropeza,Maira
dc.creatorde García,Eva
dc.date2004-12-01
dc.date.accessioned2019-05-03T12:44:10Z
dc.date.available2019-05-03T12:44:10Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582004000300010
dc.identifier.urihttp://revistaschilenas.uchile.cl/handle/2250/84795
dc.descriptionTo establish an efficient regeneration system for Anthurium andreanum cv Rubrun, seeds from plant spadixes were germinated on a medium supplemented with 2.2 muM BA. After 2 weeks, 74% of the seeds germinated and four weeks later, micro-cuttings from these plantlets were subcultured on a medium containing 4.4 muM BA and 0.05 muM NAA. On average, 3.6 shoots per explant were obtained. Four weeks old in vitro plants from germinated seeds and the plantlets obtained from micro-cuttings, showed callus proliferation at the stem base. These tissues were subcultured on a medium supplemented with 8.9 muM BA and 2.7 muM NAA. After 6 weeks of culture, about 43.8 plantlets per square cm of callus were obtained. Anatomical studies showed the organogenic nature of these calli. Anthurium andreanum plants regenerated by organogenesis were transferred to pots and a rate of 80% of plant acclimatization was obtained.
dc.formattext/html
dc.languageen
dc.publisherPontificia Universidad Católica de Valparaíso
dc.relation10.4067/S0717-34582004000300010
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceElectronic Journal of Biotechnology v.7 n.3 2004
dc.titlePlant regeneration of Anthurium andreanum cv Rubrun


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