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dc.creatorMartínez,Carolina
dc.creatorPetruccelli,Silvana
dc.creatorGiulietti,Ana María
dc.creatorAlvarez,María Alejandra
dc.date2005-08-01
dc.date.accessioned2019-05-03T12:44:12Z
dc.date.available2019-05-03T12:44:12Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000200006
dc.identifier.urihttp://revistaschilenas.uchile.cl/handle/2250/84821
dc.descriptionNicotiana tabacum hairy roots that express the antibody 14D9 were established. The 14D9 antibody yield obtained after 20 days of culture was 5.95 μg 14D9ml-1. The addition of the reticulum endoplasmic retention sequence KDEL demonstrated a positive effect over the intracellular 14D9 amounts with a yield increase up to 20.82 µg ml-1. DMSO increased the antibody amount in the biomass from 20.00 to 64.03 µg ml-1 while PVP (at 1.5 gl-1) and gelatine (at 5.0 gl-1) increased total 14D9 amounts in the culture medium to 25 µg and 14 µg respectively
dc.formattext/html
dc.languageen
dc.publisherPontificia Universidad Católica de Valparaíso
dc.relation10.4067/S0717-34582005000200006
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceElectronic Journal of Biotechnology v.8 n.2 2005
dc.subjectcatalytic antibodies
dc.subjectin vitro cultures
dc.subjectmolecular farming
dc.subjectrecombinant antibodies
dc.subjecttobacco
dc.titleExpression of the antibody 14D9 in Nicotiana tabacum hairy roots


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