dc.creator | Martínez,Carolina | |
dc.creator | Petruccelli,Silvana | |
dc.creator | Giulietti,Ana María | |
dc.creator | Alvarez,María Alejandra | |
dc.date | 2005-08-01 | |
dc.date.accessioned | 2019-05-03T12:44:12Z | |
dc.date.available | 2019-05-03T12:44:12Z | |
dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000200006 | |
dc.identifier.uri | http://revistaschilenas.uchile.cl/handle/2250/84821 | |
dc.description | Nicotiana tabacum hairy roots that express the antibody 14D9 were established. The 14D9 antibody yield obtained after 20 days of culture was 5.95 μg 14D9ml-1. The addition of the reticulum endoplasmic retention sequence KDEL demonstrated a positive effect over the intracellular 14D9 amounts with a yield increase up to 20.82 µg ml-1. DMSO increased the antibody amount in the biomass from 20.00 to 64.03 µg ml-1 while PVP (at 1.5 gl-1) and gelatine (at 5.0 gl-1) increased total 14D9 amounts in the culture medium to 25 µg and 14 µg respectively | |
dc.format | text/html | |
dc.language | en | |
dc.publisher | Pontificia Universidad Católica de Valparaíso | |
dc.relation | 10.4067/S0717-34582005000200006 | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.source | Electronic Journal of Biotechnology v.8 n.2 2005 | |
dc.subject | catalytic antibodies | |
dc.subject | in vitro cultures | |
dc.subject | molecular farming | |
dc.subject | recombinant antibodies | |
dc.subject | tobacco | |
dc.title | Expression of the antibody 14D9 in Nicotiana tabacum hairy roots | |