High performance enzymatic synthesis of oleyl oleate using immobilised lipase from Candida antartica
Author
Mat Radzi,Salina
Basri,Mahiran
Bakar Salleh,Abu
Ariff,Arbakariya
Mohammad,Rosfarizan
Abdul Rahman,Mohd. Basyaruddin
Abdul Rahman,Raja Noor Zaliha Raja
Abstract
High performance enzymatic synthesis of oleyl oleate, a liquid wax ester was carried out by lipase-catalysed esterification of oleic acid and oleyl alcohol. Various reaction parameters were optimised to obtain high yield of oleyl oleate. The optimum condition to produce oleyl oleate was reaction time; 5 min, organic solvents of log P ≥ 3.5, temperature; 40-50ºC, amount of enzyme; 0.2-0.4 g and molar ratio of oleyl alcohol to oleic acid; 2:1. The operational stability of enzyme was maintained at >90% yield up to 9 cycles. Analysis of the yield of the product showed that at optimum conditions, >95% liquid wax esters were produced