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dc.creatorSarwat,Maryam
dc.creatorSingh Negi,Madan
dc.creatorLakshmikumaran,Malathi
dc.creatorKumar Tyagi,Akhilesh
dc.creatorDas,Sandip
dc.creatorShankar Srivastava,Prem
dc.date2006-01-01
dc.date.accessioned2019-05-03T12:44:15Z
dc.date.available2019-05-03T12:44:15Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000100011
dc.identifier.urihttp://revistaschilenas.uchile.cl/handle/2250/84850
dc.descriptionFor studying genetic diversity in natural populations of Terminalia, a medicinal plant, our attempts to isolate high quality DNA using several previously reported protocols and even modifications were unsuccessful. We therefore combined CTAB based isolation, and column based purification step, to isolate DNA from Terminalia arjuna. The DNA isolated using this standardized protocol was high in quality and suitable for restriction digestion and generation of random amplification of polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP).
dc.formattext/html
dc.languageen
dc.publisherPontificia Universidad Católica de Valparaíso
dc.relation10.4067/S0717-34582006000100011
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceElectronic Journal of Biotechnology v.9 n.1 2006
dc.subjectDNA extraction
dc.subjectmedicinal plants
dc.subjectmolecular markers
dc.subjectTerminalia species
dc.titleA standardized protocol for genomic DNA isolation from Terminalia arjuna for genetic diversity analysis


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