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dc.creatorFernández R.,Marta
dc.creatorValenzuela A.,Sofía
dc.creatorBalocchi L.,Claudio
dc.date2006-06-01
dc.date.accessioned2019-05-03T12:44:19Z
dc.date.available2019-05-03T12:44:19Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300025
dc.identifier.urihttp://revistaschilenas.uchile.cl/handle/2250/84886
dc.descriptionEucalyptus globulus is the second most important forest species in Chile, after Pinus radiata. The main advantages of E. globulus are its fast growth (25 m³/ha/year) and its excellent wood quality for kraft pulp production. On the negative side, its low freezing tolerance has been an obstacle for the expansion of plantations, specifically on the foothills of the Andes. The difference in the freezing resistance between clones of E. globulus has a genetic base and, therefore, it could be detected through DNA molecular markers. Fifteen clones of E. globulus, eight classified as freezing resistant and seven as freezing sensitive were analyzed using the technique of RAPD, in order to obtain molecular markers that could differentiate between freezing sensitive and resistant clones. Eighteen primers amplified reproducible bands. Three bands were only present in freezing resistant clones, two bands of 768 bp, 602 bp obtained with UBC 218 primer and one band of 248 bp obtained with UBC 237 primer. The preliminary results indicate that polymorphism can be observed with the primers employed, but it is not possible to associate the bands with the cold resistance or susceptibility in E. globulus.
dc.formattext/html
dc.languageen
dc.publisherPontificia Universidad Católica de Valparaíso
dc.relation10.4067/S0717-34582006000300025
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceElectronic Journal of Biotechnology v.9 n.3 2006
dc.subjectEucalyptus globulus
dc.subjectfreezing resistance
dc.subjectmolecular markers
dc.subjectRAPD
dc.titleRAPD and freezing resistance in Eucalyptus globulus


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