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Optimization of culture conditions for exopolysaccharides production in Rhizobium sp. using the response surface method

Author
Pereira Duta,Flávia

Pessôa de França,Francisca

de Almeida Lopes,Léa Maria

Full text
https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000400007
Abstract
The combined effects of the processing parameters for exopolysaccharides production by Rhizobium sp. was studied using the experimental design and response surface methodology. The experiments were carried out using a fermenter with 20 L capacity, as the reactor. All processing parameters were online monitored. The temperature [(30 ± 1)ºC] and pH value (7.0 ± 0.1) were kept constant throughout the experimental time. As statistical tools, a complete 2³ factorial planning with central point and response surface were used to study the interactions among three relevant variables of the fermentation process: calcium carbonate concentration, aeration and agitation. The processing parameters setup for reaching a maximum response for exopolysaccharides production was obtained when applying the highest values for calcium carbonate concentration (1.1 g/L), aeration (1.3 vvm) and agitation (800 rpm). In addition, the combination of these optimum processing parameters yielded Y P/S (g/g) = 0.35.
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Discipline
Artes, Arquitectura y UrbanismoCiencias Agrarias, Forestales y VeterinariasCiencias Exactas y NaturalesCiencias SocialesDerechoEconomía y AdministraciónFilosofía y HumanidadesIngenieríaMedicinaMultidisciplinarias
Institutions
Universidad de ChileUniversidad Católica de ChileUniversidad de Santiago de ChileUniversidad de ConcepciónUniversidad Austral de ChileUniversidad Católica de ValparaísoUniversidad del Bio BioUniversidad de ValparaísoUniversidad Católica del Nortemore

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